Anti gm130 12480

The following antibodies were purchased: anti-phospho-AKT (S473) D9E #4060, anti-AKT #9272, anti-Caspase-3 #9662, anti-LC3 A/B (CST#4108), anti-vimentin #5741, anti-GM130 #12480, anti-phospho p44/42 MAPK (T202/Y204) #4370s, anti-LC3 #4108 (used for western blotting) from Cell Signaling Technology (Leiden, The Netherlands); anti-E-cadherin #610182 from BD Biosciences (San Jose, CA, USA); anti-calnexin #SPC-108 from Stress Marq Biosciences Inc. (Victoria, Canada); anti-CD107a/Lamp1 (clone H4A3) #SAB4700416, anti-αTubulin (clone B512) #T5168, anti-γtubulin #T6657 from Sigma-Aldrich (St. Louis, MI, USA); anti-GOLGIN-97 #A-21270 Thermo Fisher Scientific—Invitrogen, (Carlsbad, CA, USA); anti-LC3 (clone 5F10) #0231, used for immunofluorescence, from Nanotools (Teningen, Germany); anti-PFKM #a5477 from Abclonal Technology (Woburn, MA, USA); anti-PARP #sc-7150 from Santa Cruz Biotechnology, Inc (Santa Crus, CA, USA). Alexa-Fluor (488 and 546) secondary antibodies A11029, A11030, A11034, and A11035, were from Thermo Fisher Scientific—Invitrogen, (Carlsbad, CA, USA) and horseradish peroxidase (HRP)-conjugated secondary antibodies used for Western blot analyses were from Amersham Pharmacia (Buckinghamshire, UK).

Cells were lysed in RIPA buffer and subjected to western blot analysis as described previously 51 (link). Anti-Cox2 (ab198286), anti-MFN2 (ab56889), anti-SDHB (ab14714), anti-P4HB (ab137110), anti-PMP70 (ab3421), anti-PGC-1α (ab191838), anti-TFAM (ab272885), and anti-NRF1 (ab175932) antibodies were purchased from Abcam (Cambridge, UK). An anti-LC3B (#L10382) antibody was purchased from Invitrogen (Waltham, MA, USA). Anti-ULK1 pS555 (#5869), anti-ULK1 pS317 (#37762), anti-ULK1 (#8054), and anti-GM130 (#12480) antibodies were purchased from Cell Signaling Technology (Danvers MA, USA). An anti-OPA1 (#612606) antibody was purchased from BD Biosciences (San Jose, CA, USA). An anti-PINK1 (#BC100-494) antibody was purchased from Novus Biologicals (Littleton, CO, USA). Anti-Tom20 (SC-11415) and anti-Actin (SC-47778) antibodies were purchased from Santa Cruz (Dallas, TX, USA). All western blot analyses were repeated three times. Band intensities were quantified using densitometry and ImageJ software (NIH).