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2 protocols using 6 hydroxy 2 5 7 8 tetramethylchromane 2 carboxylic acid trolox gallic acid

1

Antioxidant Capacity Determination

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Folin-Ciocalteu reagent, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,4,6-tris(2-pyridyl)-s-triazine (≥99.0%) were supplied from Sigma-Aldrich (Steinheim, Germany). 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammoniumsalt (98%) was purchased from J&K, Scientific Ltd. (Beijing, China). Additionally, sodium carbonate anhydrous and ferric chloride hexahydrate were supplied from Centrohem (Stara Pazova, Serbia), while acetic acid (99.8%) and potassium peroxydisulfate were purchased from Lach-Ner (Neratovice, Czech Republic). Sodium acetate anhydrous was purchased from Kemika (Zagreb, Croatia). Ultra-pure water was obtained from a Milli-Q Plus system (EMD Millipore, Billerica, MA, USA). All other chemicals used were of analytical reagent grade.
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2

Antioxidant Assay Protocol

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Folin-Ciocalteu phenolic reagent, anhydrous sodium carbonate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium acetate trihydrate, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), ferric chloride hexahydrate, quercetin, potassium persulfate, and 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), gallic acid, 2,2-azino-bis(3-ethylbenzothiazoline-6sulfonic acid) (ABTS) were obtained from Sigma-Aldrich products (St. Louis, Missouri, USA). Aluminum chloride and potassium acetate were acquired from Fermont S.A. de C.V. and Meyer S.A. de C.V., Mexico, respectively. All reagent were analytical grade.
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