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Fab9333g

Manufactured by R&D Systems
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The FAB9333G is a laboratory instrument designed for performing analytical tasks. It is a versatile piece of equipment that can be utilized in various research and testing applications. The core function of the FAB9333G is to provide accurate and reliable data analysis.

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Lab products found in correlation

Fab9332r, by R&D Systems (2 mentions) Ic003g, by R&D Systems (2 mentions) Mouse serum igg, by Merck Group (2 mentions) Ic003r, by R&D Systems (2 mentions) Lsr 2 flow cytometer, by BD (2 mentions) Af 488 mouse anti human ace2, by R&D Systems (1 mentions)

2 protocols using fab9333g

1

Quantifying Human ACE2 Expression

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Cells were blocked with mouse serum IgG (Sigma, 15381) for 10 min at room temperature and then incubated with specific antibodies; AF-647 mouse anti-human ACE2 (Clone # 535919) (R&D systems, FAB9332R), AF-488 mouse anti-human ACE2 (Clone # 171607) (R&D systems, FAB9333G) (0.4 µg/106 cells), AF-647 isotype control mouse IgG2b (Clone # 20102) (R&D systems, IC003R) or AF-488 isotype control mouse IgG2bAF488 (Clone # 20102) (R&D systems, IC003G) for 45 min on ice, followed by washing twice with 2% EV-free FBS/PBS. Finally, the cells were diluted in 2% EV-free FBS/PBS and analyzed on a BD-LSR II flow cytometer (BD Biosciences). Data were analyzed by BD FACSDiva softwares v8.0.2 or v8.0.3 or Flow Jo v10.6.2.
El-Shennawy L., Hoffmann A.D., Dashzeveg N.K., McAndrews K.M., Mehl P.J., Cornish D., Yu Z., Tokars V.L., Nicolaescu V., Tomatsidou A., Mao C., Felicelli C.J., Tsai C.F., Ostiguin C., Jia Y., Li L., Furlong K., Wysocki J., Luo X., Ruivo C.F., Batlle D., Hope T.J., Shen Y., Chae Y.K., Zhang H., LeBleu V.S., Shi T., Swaminathan S., Luo Y., Missiakas D., Randall G.C., Demonbreun A.R., Ison M.G., Kalluri R., Fang D, & Liu H. (2022). Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2. Nature Communications, 13, 405.
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2

ACE2 Expression Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were blocked with mouse serum IgG (Sigma, 15381) for 10 min at room temperature and then incubated with specific antibodies; AF-647 mouse anti-human ACE2 (R&D systems, FAB9332R), AF-488 mouse anti-human ACE2 (R&D systems, FAB9333G), AF-647 isotype control mouse IgG2b (R&D systems, IC003R) or AF-488 isotype control mouse IgG2bAF488 (R&D systems, IC003G) for 45 min on ice, followed by washing twice with 2% exosome-free FBS/PBS. Finally, the cells were diluted in 2% exo-free FBS/PBS and analyzed on a BD-LSR II flow cytometer (BD Biosciences).
El-Shennawy L., Hoffmann A.D., Dashzeveg N.K., Mehl P.J., Yu Z., Tokars V., Nicolaescu V., Ostiguin C., Jia Y., Li L., Furlong K., Mao C., Wysocki J., Batlle D., Hope T.J., Shen Y., Luo Y., Chae Y.K., Zhang H., Swaminathan S., Prescott M., Demonbreun A.R., Ison M.G., Fang D., & Liu H. (2020). Circulating ACE2-expressing Exosomes Block SARS-CoV-2 Infection as an Innate Antiviral Mechanism.
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