The right lung of animals was homogenized in five volumes of 50 mM phosphate buffer solution (pH 7.0), and the resulting supernatant was used for analysis after centrifugation (Micro 17R, Hanil Science). Catalase (Cayman catalase kit 707002, Cayman Chemical, Ann Arbor, MI, USA), reduced glutathione (GSH) (Cayman glutathione assay kit 703002, Cayman Chemical), and thiobarbituric acid reactive substance (TBARS) (Cayman TBARS assay kit 10009055, Cayman Chemical) were measured in the supernatant following adjustment to a final protein concentration of 2 mg/mL.
Cayman tbars assay kit 10009055
Manufactured by Cayman Chemical
Sourced in United States
The Cayman TBARS Assay Kit 10009055 is a colorimetric assay designed to measure thiobarbituric acid reactive substances (TBARS) in various sample types. The kit provides the necessary reagents and protocols to quantify TBARS, which are used as biomarkers of oxidative stress.
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Lab products found in correlation
2 protocols using cayman tbars assay kit 10009055
1
Antioxidant Enzyme Evaluation in Lung Tissue
2
Oxidative Stress Biomarker Analyses
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Oxidative stress analyses were performed in lysate red blood cells and/or plasma according to the manufacturer’s instructions. Total antioxidant capacity (Cayman Antioxidant Assay kit 709001, Cayman Chemical, 1180 East Ellsworth Road Ann Arbor, Michigan 48108 USA) [38 (link),39 (link)], reduced glutathione (GSH) (Cayman glutathione assay kit 703002, Cayman Chemical) [40 (link),41 (link)], and thiobarbituric acid reactive substance (TBARS) (Cayman TBARS assay kit 10009055, Cayman Chemical, 1180 East Ellsworth Road Ann Arbor, Michigan 48108 USA) [38 (link),39 (link)] were evaluated. Reactive oxygen species (ROS) level analysis was performed accordingly with method described by Tsamesidis et al. [14 (link)].
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