Mms19 16015 1 ap

Manufactured by Proteintech

The MMS19 (16015-1-AP) is a polyclonal antibody produced by Proteintech. It is designed for the detection of the MMS19 protein in various biological samples using techniques such as Western blotting, immunohistochemistry, and immunofluorescence.

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Lab products found in correlation

Anti flag antibody, by OriGene (1 mentions) Ab96488, by Abcam (1 mentions) Ab83088, by Abcam (1 mentions) Anti mouse hrp na 931, by Cytiva (1 mentions) Mip18 fam96b 20108 1 ap, by Proteintech (1 mentions) Fusionsolo, by WITec (1 mentions)

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MMS19 (2 citations)

2 protocols using mms19 16015 1 ap

Antibody Validation for Proteomic Analysis

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Antibodies in this study were as follows: anti-HSC20 westerns were performed either with a custom-made antibody (Genscript) or with a commercial antibody (Sigma #HPA018447). Anti-CIAO1 (sc-374498) and NFS1 (sc-81107) were from Santa Cruz Biotechnology. Anti-nsp13 (NBP2-89168) was from Novus Biologicals. Anti-HSPA9 (HPA000898) was from Sigma. Anti-FAM96B (20108-1-AP) and MMS19 (16015-1-AP) were from Proteintech. Anti-FLAG antibody was from Origene (TA50011). Anti-Strep II (ab184224) was from Qiagen (1023944).

Maio N., Raza M.K., Li Y., Zhang D.L., Bollinger JM J.r., Krebs C, & Rouault T.A. (2023). An iron–sulfur cluster in the zinc-binding domain of the SARS-CoV-2 helicase modulates its RNA-binding and -unwinding activities. Proceedings of the National Academy of Sciences of the United States of America, 120(33), e2303860120.

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Western Blot Antibody Validation Protocol

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Western blots were imaged on a FUSION SOLO (Witec AG) chemiluminescence imaging system. The following primary antibodies (at a dilution of 1:1000) were used: β-actin-horseradish peroxidase (HRP) (C4, sc-47778; Santa Cruz Biotechnology), DNA2 (ab96488; Abcam), CIAO1 (ab83088; Abcam), MIP18/FAM96b (20108-1-AP; Proteintech), MMS19 (16015-1-AP; Proteintech). Secondary antibodies (at a dilution of 1: 2500) were anti-Mouse-HRP (NA931; Amersham) and anti-Rabbit-HRP (NA934; Amersham). Uncropped blots can be found in Supplementary Fig. 6.

Mariotti L., Wild S., Brunoldi G., Piceni A., Ceppi I., Kummer S., Lutz R.E., Cejka P, & Gari K. (2020). The iron–sulphur cluster in human DNA2 is required for all biochemical activities of DNA2. Communications Biology, 3, 322.

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