Sc 374463

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Sc-374463 is a laboratory instrument designed for the detection and analysis of molecular compounds. It utilizes advanced spectroscopic techniques to provide accurate and reliable data. The core function of this product is to facilitate the identification and quantification of specific molecules within complex samples.

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Lab products found in correlation

Lsm 880, by Zeiss (4 mentions) Ab175404, by Abcam (2 mentions) Ab92511, by Abcam (2 mentions) Ab9498, by Abcam (2 mentions) Pvdf western blotting membrane, by Roche (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Sc 47778, by Santa Cruz Biotechnology (1 mentions) Sc 390351, by Santa Cruz Biotechnology (1 mentions) Phototope hrp western blot detection kit, by New England Biolabs (1 mentions) Ab134114, by Abcam (1 mentions) Ab8878, by Abcam (1 mentions) Ab135372, by Abcam (1 mentions) Decorin, by Abcam (1 mentions)

3 protocols using sc 374463

Western Blot Analysis of Protein Expression

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Protein expression in the samples was analysed by western blotting. The total protein lysate was extracted with cell lysis buffer for western blotting and immunoprecipitation (No. P0013, Beyotime) and denatured by boiling. Protein samples were resolved on 12 % SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF Western Blotting Membranes, Roche). Membranes were blocked in PBS containing 5 % (w/v) non-fat dry milk and 0.1 % Tween-20 for 2 h, incubated with the appropriate antibodies overnight, washed with TBST buffer, and then incubated with secondary antibodies for 2 h. Blots were developed using a horseradish peroxidase-linked secondary antibody and developed with a chemiluminescent detection system (Phototope-HRP Western blot detection kit, New England Biolab).
The primary antibodies used for blotting were as follows: anti-Smad5 (1:1000, 12534, Cell Signaling Technology), anti-p-Smad5 (1:1000, 9516S, Cell Signaling Technology), anti-OGN (1:1000, sc-374463, Santa Cruz), anti-Runx2 (1:1000, sc-390351; Santa Cruz), and anti-β-actin (1:1000, sc-47778, Santa Cruz).

Zhang W., Chen L., Wu J., Li J., Zhang X., Xiang Y., Li F., Wu C., Xiang L., Ran Q, & Li Z. (2019). Long noncoding RNA TUG1 inhibits osteogenesis of bone marrow mesenchymal stem cells via Smad5 after irradiation. Theranostics, 9(8), 2198-2208.

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Immunostaining of Human Periodontal Tissues

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Human periodontal soft tissue biopsies were stained with H&E for morphological evaluation. For fluorescent immunostaining, human periodontal soft tissue biopsies were incubated with primary antibodies diluted in 3% bovine serum albumin (BSA)/phosphate-buffered saline (PBS) overnight at 4°C. Primary antibodies used include decorin (1:50, ab175404, Abcam, Cambridge, UK), osteoglycin (1:50, sc-374463, Santa Cruz Biotechnology, Dallas, TX, USA), HLA-DR (1:50, ab92511, Abcam), CD11b (1:50, ab8878, Abcam), CD3 (1:50, ab135372, Abcam), CD19 (1:50, ab134114, Abcam), CXCL13 (1:100, PA5-47035, Invitrogen, Waltham, MA, USA), and CD31 (1:50, ab9498, Abcam). Next-day samples were incubated in Alexa-fluor 488, 594 Monkey anti-Mouse, Alexa-fluor 647 Monkey anti-Rabbit, or Alexa-fluor 488 Monkey anti-Goat secondary antibodies (Jackson ImmunoResearch, Jackson, PA, USA). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Images were acquired using Zeiss LSM 880. Samples were evaluated in a blinded fashion at two to three different levels of sectioning according to the staining extent and intensity.

Qian S.J., Huang Q.R., Chen R.Y., Mo J.J., Zhou L.Y., Zhao Y., Li B, & Lai H.C. (2021). Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis. Frontiers in Immunology, 12, 711337.

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Immunohistochemical Analysis of Periodontal Tissues

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Human periodontal soft tissue biopsies were incubated with primary antibodies diluted in 3% BSA/PBS overnight at 4℃. Primary antibodies used include Decorin (1:50, ab175404, Abcam), Osteoglycin (1:50, sc-374463, Santa Cruz), HLA-DR (1:50, ab92511, Abcam), and CD31 (1:50, ab9498, Abcam). Next day samples were incubated in Alexa-fluor 488, 594 Goat anti-Mouse or Goat anti-Rabbit secondary antibodies (Jackson Immunoresearch). Nuclei were counterstained with DAPI. Pictures were acquired using Zeiss LSM 880.

Qian S., Huang Q., Shi J., Zhou L., Zhao Y., Li B., & Lai H. (2020). Single-cell RNA sequencing identifies heterogeneous cell subtypes within gingival tissue.

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