Pro collagen iα1 elisa kit

Manufactured by R&D Systems

Sourced in United States

The Pro-collagen Iα1 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed to measure the concentration of pro-collagen Iα1 in biological samples. The kit utilizes a specific antibody coated on a microplate to capture the target analyte from the sample. After a series of washes, a detection antibody is added, and a colorimetric reaction is developed to quantify the amount of pro-collagen Iα1 present.

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Lab products found in correlation

Bio plex pro human cytokine grp 1 panel 27 plex assay, by Bio-Rad (2 mentions) Sircol collagen assay, by Biocolor (2 mentions)

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ELISAs (144 citations)

2 protocols using pro collagen iα1 elisa kit

Soluble Factors in Pancreatic Cancer Fibrosis

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Co-culture experiments were carried out with B cells and B-cell subsets from patients with PDAC, primary human pancreatic fibroblasts, and CAF. Polycarbonate semipermeable membranes (Nunc Dominique Dutscher, Brumath, France) were used to investigate soluble factors or contact-mediated pro-fibrotic mechanisms. Soluble collagen concentration in the supernatant of fibroblasts/CD19 + B cells co-cultures was evaluated using Sircol collagen assay (Biocolor Ltd. Interchim, Montluçon, France). Soluble collagen concentration in the supernatant of fibroblasts/B-cells subsets co-cultures was evaluated using the Pro-collagen Iα1 ELISA kit (R&D Systems Inc., Minneapolis, MN, USA). Cytokines and chemokines in the supernatant of fibroblasts/CD19 + B cells co-cultures were measured using the Bio-Plex Pro Human Cytokine Grp I Panel 27-plex assay (Bio-Rad, Hercules, CA, USA). A detailed description of the methods used for the establishment of primary human pancreatic fibroblasts lines and of co-cultures with total B cells and B-cell subsets is provided in the Methods section of this article’s Online Repository.

Minici C., Rigamonti E., Lanzillotta M., Monno A., Rovati L., Maehara T., Kaneko N., Deshpande V., Protti M.P., De Monte L., Scielzo C., Crippa S., Arcidiacono P.G., Dugnani E., Piemonti L., Falconi M., Pillai S., Manfredi A.A, & Della-Torre E. (2020). B lymphocytes contribute to stromal reaction in pancreatic ductal adenocarcinoma. Oncoimmunology, 9(1), 1794359.

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Fibrotic Mechanisms in IgG4-RD

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Co-culture experiments were carried out with B cells and B-cell subsets from patients with IgG4-RD AIP, and primary human pancreatic or skin fibroblasts. Polycarbonate semipermeable membranes (Nunc Dominique Dutscher, Brumath, France) were used to identify soluble factors or contact mediated pro-fibrotic mechanisms. Soluble collagen concentration in the supernatant of fibroblasts/CD19+ B cells co-cultures was evaluated using Sircol collagen assay (Biocolor Ltd. Interchim, Montluçon, France). Soluble collagen concentration in the supernatant of fibroblasts/B-cells subsets co-cultures was evaluated using the Pro-collagen Iα1 ELISA kit (R&D Systems Inc., Minneapolis, MN, USA). Cytokines and chemokines present in the supernatant of fibroblasts/CD19+ B cells co-cultures were measured using the Bio-Plex Pro Human Cytokine Grp I Panel 27-plex assay (Bio-Rad, Hercules, CA, USA). A detailed description of the methods used for the establishment of primary human pancreatic fibroblasts cell lines and co-cultures with total B cells and B-cell subsets is provided in the Methods section of this article’s Online Repository.

Della-Torre E., Rigamonti E., Perugino C., Sain S.B., Sun N., Kaneko N., Maehara T., Rovati L., Ponzoni M., Milani R., Lanzillotta M., Mahajan V., Mattoo H., Molineris I., Deshpande V., Stone J.H., Falconi M., Manfredi A.A, & Pillai S. (2019). B lymphocytes directly contribute to tissue fibrosis in IgG4-Related Disease. The Journal of allergy and clinical immunology, 145(3), 968-981.e14.

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