Dexamethasone (dex)

Manufactured by Topscience

Sourced in China

Dexamethasone is a synthetic glucocorticoid medication used as an anti-inflammatory and immunosuppressant agent. It is a potent steroid drug that reduces inflammation and suppresses the immune system.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Sodium β glycerophosphate, by Merck Group (1 mentions) α mem, by Thermo Fisher Scientific (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Metformin, by Beyotime (1 mentions) 3 isobutyl 1 methyl xanthine (ibmx), by Topscience (1 mentions) Gentlemacs, by Miltenyi Biotec (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

3 protocols using dexamethasone (dex)

Inflammatory Response Modulation in RAW264.7 Cells

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RAW264.7 cells (FuHeng) were cultured in Dulbecco's modified Eagle's medium (DMEM; Thermo Scientific) supplemented with 10% fetal bovine serum (FBS; Thermo Scientific) and 1% penicillin/streptomycin in an incubator (37°C, 5% CO₂). The cells were treated with 10% FBS/DMEM for 24 h before administration. Concentrations of LPS, cytosporone B (Csn‐B), and Dex were prepared as described.²⁵, ²⁶, ²⁷, ²⁸, ²⁹, ³⁰ Dex preconditioning was used based on the current evidence of its potent anti‐inflammatory effect.²⁸, ²⁹ Both the LPS group and the Dex + LPS group were treated with 500 ng/mL LPS (Sigma) for 3 h, while the control group was not treated with LPS. Before LPS induction, the agonist group was pretreated with 10 g/mL Csn‐B (MCE) for 24 h, and the DMSO group was used as vehicle control, while the Dex + LPS and Dex groups were pretreated with 10 μM Dex (Topscience) for 30 min. For all experiments, the cells were seeded in six‐well plates at a density of 1.0 × 10⁶ cells/mL and incubated for 24 h.

Zhang Q., Huang Y., Gong C., Tang Y., Xiong J., Wang D, & Liu X. (2023). Dexmedetomidine attenuates inflammation and organ injury partially by upregulating Nur77 in sepsis. Immunity, Inflammation and Disease, 11(6), e883.

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Osteogenic Differentiation of BMSCs

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BMSCs were seeded in a 12‐well plate and cultured in α‐minimum essential medium (α‐MEM; Gibco, Grand Island, NY, USA) containing 10% FBS (Gibco), 10 mM sodium β‐glycerophosphate (Merck KGaA, Darmstadt, Germany), 250 μM ascorbic acid (Merck KGaA), and 100 nM dexamethasone (Topscience, Shanghai, China). After a seven‐day culture, the cells were subjected to alkaline phosphatase (ALP) staining and ALP activity examination. Protein expression levels of ossification marker genes were determined on the 14th day, and alizarin red staining was performed on the 21st day of culture.

Zhou G., Yan X., Chen Z., Zeng X, & Wu F. (2023). ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic Differentiation of Bone Marrow Mesenchymal Stromal Cells and Extracellular Matrix Mineralization of Osteoblasts. Orthopaedic Surgery, 15(9), 2423-2434.

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Isolation and Cultivation of Human Adipocytes

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Human adipocytes were acquired from fresh omentum of ovarian cancer patients undergoing surgery. Fresh omentum was minced with scissors, digested by gentleMACS (Miltenyi) [45 (link)] to obtain single cells. After centrifuge with condition of 100rpm, the suspension separated into 4 layers, which are cell pallets, conditional medium, adipocytes and free fat oil. And then we carefully isolated adipocytes, counted and cultured in six-well plates with DF12 (Gibco) supplemented with 2% FBS (Gibco) for 1×10⁶ adipocytes. 48 hours later, the supernatant was harvested as conditioned medium of human primary cultured adipocytes (hCM). hCM were filtered through 200-mesh sieves to remove cell debris before use.
3T3-L1 cells were chemically induced by DMEM with 10% FBS (Gibco), 0.5mM IBMX (TOPSCIENCE, T1713), 1μM Dexamethasone (TOPSCIENCE, T1076), 5μg/ml insulin (Bovine, Sigma I-5500) for 2 days and DMEM with 10% FBS (Gibco), 10μg/ml insulin (Bovine, Sigma I-5500) for 2 days to differentiate into adipocyte-like cells. Then the supernatant of induced adipocyte-like 3T3-L1 cells was collected at 24 hours as mouse conditioned medium (mCM). After pretreatment with metformin (1 mM, Beyotime, catalog number s1741–1g) for 12 hours, the induced adipocyte-like 3T3-L1 cells were cultured with complete medium for 24 hours, and the supernatant collected as CM (MET).

Sun C., Li X., Guo E., Li N., Zhou B., Lu H., Huang J., Xia M., Shan W., Wang B., Li K., Weng D., Xu X., Gao Q., Wang S., Hu J., Lu Y., Mills G.B, & Chen G. (2019). MCP-1/CCR-2 axis in adipocytes and cancer cell respectively facilitates ovarian cancer peritoneal metastasis. Oncogene, 39(8), 1681-1695.

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