Ribonucleotide triphosphates

All oligonucleotides were purchased from Integrated DNA Technologies (IDT, Skokie, IL, USA). T4 ligase was obtained from Promega (Madison, WI, USA). T7 RNA polymerase and ribonucleotide triphosphates (rNTPs) were purchased from New England BioLabs (NEB, Ipswich, MA, USA). Zeba^TM spin desalting columns were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Cyanine 5-UTP was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Mica and Lacey Formvar/carbon-coated copper grids (01883-F) were obtained from Ted Pella (Redding, CA, USA). Dulbecco’s modified Eagle’s medium (DMEM), penicillin/streptomycin (P/S), fetal bovine serum (FBS), and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Gibco (Waltham, MA, USA). The Stemfect^TM RNA Transfection Kit was purchased from Stemgent (Houston, TX, USA). CelLytic M and MgCl₂ solution were purchased from Sigma-Aldrich (Saint Louis, MI, USA). A Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Laboratories (Kumamoto, Japan). Tris-HCl buffer was purchased from Invitrogen (Waltham, MA, USA).

Enzymes, ribonucleotide triphosphates (NTPs) and gel loading buffers were purchased from New England Biolabs (NEB) unless noted otherwise. DFHBI-1T was purchased from Lucerna. All other chemicals used were of molecular biology grade or equivalent, or of the highest quality available, and purchased from Nacalai Tesque or Wako Pure Chemical Co. The 1 × reaction buffer used in this work has the following composition: 40 mM Tris–HCl, pH 7.9, 10 mM MgCl₂, 1 mM dithiothreitol (DTT), 2 mM spermidine, 100 mM KCl.