Hup t3

HT29, MIAPaCa-2, CFPAC1, and RKO were purchased from ATCC. DLD1 were obtained from Sigma, while SW620 and MDST8 were purchased from European Collection of Animal Cell Cultures and HUPT3 obtained from DSMZ. All cell lines were authenticated by short tandem repeat profiling analysis. They were routinely subjected to Mycoplasma testing and used up to 20 passages. HT29, MIAPaCa-2, CFPAC1, SW620, and MDST8 were maintained in DMEM (#11960, Life Technologies) supplemented with 10% FBS (#S181H-100, Biowest) and 2 mmol/L Glutamine (#AA29168, Life Technologies), while DLD1 were maintained in RPMI (#31870, Life Technologies) supplemented with 10% FBS and 2 mmol/L Glutamine and HUPT3 was cultured in MEMa 90% with 10% FBS, 2 mmol/L Glutamine; 1% nonessential amino acids; 1% sodium pyruvate. Doxycycline was purchased from Clontech (#631311) and dissolved in sterile water and filtered to make 1 mg/mL stock solutions. TGFβ recombinant human protein was purchased from Thermo Fisher Scientific (#CTP9211) and dissolved in sterile water to make 0.1 mg/mL stock solutions. Aliquots of the stock solutions were stored at −20°C.