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2 protocols using soy peptone

1

Characterization of Organic Compounds

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All solvents used were of reagent grade. Tryptone tryptophan medium, beef extract powder, peptone bacteriological, and soy peptone were purchased from Biolife. Agar and yeast extract were purchased from Fluka Analytical. Sodium chloride, D (+)-glucose, and di potassium hydrogen phosphate trihydrate were purchased from Merck. Dulbecco's modified Eagle's medium, (DMEM), fetal bovine serum, glutamine and trypsin were purchased from Gibco, Glasgow, UK. Phosphate buffer saline (PBS) was purchased from Sigma-Aldrich. Melting points were measured in open tubes with Stuart Scientific apparatus and are uncorrected. IR spectra in the region of 4000-370 cm -1 were obtained using a Cary 670 FTIR spectrometer, Agilent Technologies. A UV-1600 PC series spectrophotometer of VWR was used to obtain electronic absorption spectra. The 1 H NMR spectra were recorded using a Bruker AC 250, 400 MHFT-NMR instrument in DMSO-d 6 . ESI-MS spectra were recorded with an Agilent 1100/LC-MS system. 119 Sn Mössbauer spectra were recorded at various sample tempera-
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2

Spectroscopic Analysis of Organic Compounds

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All solvents used were of reagent grade. Tryptone and soy peptone were purchased from Biolife, Milano, Italy. Agar was purchased from Sigma-Aldrich St. Louis, MO, USA product of Spain. Sodium cloride, D(+)–Glucose, and di potassium hydrogen phosphate trihydrate were purchased from Merck, Darmstadt, Germany. DMSO was purchased from Riedel–de Haen (Seelze, Germany). Melting points were measured in open tubes with a Stuart Scientific apparatus and are uncorrected. A UV–1600 PC series spectrophotometer of VWR international GmbH, Darmstadt, Germany was used to obtain electronic absorption spectra. ATR-FTIR spectra in the region of 4000–370 cm–1 were obtained with a Cary 670 FTIR spectrometer, Agilent Technologies Agilent Technologies. XRF measurement was carried out using an Am–241 radio isotopic source (exciting radiation 59.5 keV).
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