Anti-GLUT4, anti-phospho-AKT (Thr308), and anti-ACTB antibodies were purchased from Abcam (Cambridge, MA, USA); anti-phospho-AKT (Ser473) and anti-AKT antibodies were from Cell signaling (Beverly, MA, USA); anti-MAC2 antibody was from Cedarlane (Ontario, Canada); and rabbit IgG antibody was from Vector Laboratories (Burlingame, CA, USA). Goat anti-rabbit Alexa Fluor 488 secondary antibody was from Life technologies (Carlsbad, CA, USA); goat anti-mouse for ACTB—or otherwise donkey anti-rabbit secondary antibodies—were from LI-COR (Lincoln, NE, USA).
Anti phospho akt thr308
Manufactured by Abcam
Sourced in United States, United Kingdom
Anti-phospho-AKT (Thr308) is a primary antibody that recognizes AKT protein phosphorylated at threonine 308. It is used for the detection and quantification of AKT phosphorylated at this specific site.
Automatically generated - may contain errors
Lab products found in correlation
Anti mac2 antibody, by Cedarlane (1 mentions)
Alexa fluor 488 goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
Anti glut4, by Abcam (1 mentions)
Nitrate nitrite colorimetric assay kit, by Cayman Chemical (1 mentions)
Anti nf κb p65, by Santa Cruz Biotechnology (1 mentions)
P coumaric acid, by Merck Group (1 mentions)
Anti actin antibody, by Santa Cruz Biotechnology (1 mentions)
Luminol, by Merck Group (1 mentions)
Phosstop, by Roche (1 mentions)
17 estradiol, by Merck Group (1 mentions)
Anti inos, by Santa Cruz Biotechnology (1 mentions)
Anti glut1, by Santa Cruz Biotechnology (1 mentions)
Anti glut4, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti phospho akt thr308
1
Antibody Immunodetection Assay
2
Evaluating Estrogenic Modulation in Vitro
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ether was purchased from Lek (Ljubljana, Slovenia). Luminol, p-coumaric acid and 17ß-Estradiol (E 2 ; E8875) were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA).
Protease inhibitor (Complete, Ultra Mini, EDTA-free) and phosphatase inhibitor cocktails (PhosStop) were obtained from Roche (Mannheim, Germany). The Nitrate/Nitrite Colorimetric Assay Kit was obtained from Cayman Chemical (Ann Arbor, MI, USA). Anti phospho-Akt (Thr 308 ), and anti total Akt antibodies were obtained from Abcam (Cambridge, UK), while the ERα, ERβ, anti-iNOS, anti-NFκB-p65, anti-GLUT1, anti-GLUT4, anti-FAT/CD36 polyclonal antibodies, and the mouse monoclonal anti-actin antibody were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Secondary anti-rabbit and anti-mouse IgG
Protease inhibitor (Complete, Ultra Mini, EDTA-free) and phosphatase inhibitor cocktails (PhosStop) were obtained from Roche (Mannheim, Germany). The Nitrate/Nitrite Colorimetric Assay Kit was obtained from Cayman Chemical (Ann Arbor, MI, USA). Anti phospho-Akt (Thr 308 ), and anti total Akt antibodies were obtained from Abcam (Cambridge, UK), while the ERα, ERβ, anti-iNOS, anti-NFκB-p65, anti-GLUT1, anti-GLUT4, anti-FAT/CD36 polyclonal antibodies, and the mouse monoclonal anti-actin antibody were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Secondary anti-rabbit and anti-mouse IgG
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