Gen5 microplate reader and imaging software

Manufactured by Agilent Technologies

Sourced in United States

The Gen5 Microplate Reader and Imaging Software is a lab equipment product from Agilent Technologies. It is a microplate reader and imaging system used for various analytical applications in life science research and clinical diagnostics.

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Lab products found in correlation

Elx800 absorbance microplate reader, by Agilent Technologies (1 mentions) Nucblue fixed cell readyprobes reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Microplate reader (6925 citations) Gen5 microplate reader (129 citations) Gen5TM (34 citations) Gen5TM software (15 citations) Gen5 microplate reader software (12 citations) Gen5 imaging software (7 citations) Gen5 reader software (3 citations)

2 protocols using gen5 microplate reader and imaging software

Serum Biomarker Quantification by ELISA

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Whole blood samples were collected from participants in the morning after at least 8 hours of fasting. Serum samples from whole blood were centrifuged after coagulation and then stored at −80°C until analysis. Enzyme-linked immunosorbent assay (ELISA) analysis was performed using an ELx800 Absorbance Microplate Reader (BioTek Instruments, Winooski, VT, USA) detection system using a human ADRB3 ELISA Kit (SinoGeneClon Biotech, Hangzhou, China, Cat. No: SG-16339), a human GEF ELISA kit (SinoGeneClon Biotech, China, Cat. No: SG-16405), and a human ROCK2 ELISA Kit (SinoGeneClon Biotech, China, Cat. No: SG-10439) following the manufacturer’s protocol with sensitivity of 0.1 ng/mL, 0.1 ng/mL, and 2 pg/mL, respectively. The intra-assay and inter-assay coefficients of variation of all 3 kits were <8% and <10%, respectively. The results were analyzed using a Gen5 Microplate Reader and Imaging Software (BioTek Instruments, Winooski, VT, USA).

Fırat E., Aybek Z, & Aybek H. (2022). ADRB3, ROCK2, and GEF Levels in Overactive Bladder Patients. International Neurourology Journal, 26(Suppl 1), S8-14.

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Quantifying HSV-2 Infection Dynamics in HeLa Cells

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70–80% confluent HeLa cells were infected with HSV-2 333-GFP at 0.1 MOI. After 2 hours post infection (hpi), the media was changed to DMEM, and a drop of NucBlue Fixed Cell ReadyProbes Reagent (Thermo Fisher Scientific) was added to each well to stain the nuclei of the cells. At various time points post infection (every 4 h from 8–28 hpi), the cells were imaged using a Lionheart LX automated microscope (BioTek Instruments LLX). DAPI and GFP channels were merged to create images with the cellular nuclei (blue) and virus (green) overlaid in the same frame. The images were quantified using the BioTek Gen5 Microplate Reader and Imaging software.

Patil C.D., Suryawanshi R., Ames J., Koganti R., Agelidis A., Kapoor D., Yadavalli T., Koujah L., Tseng H.C, & Shukla D. (2021). Intrinsic antiviral activity of Optineurin prevents hyperproliferation of a primary herpes simplex virus type-2 infection. Journal of immunology (Baltimore, Md. : 1950), 208(1), 63-73.

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