Hek blue2 cells

Human lung (Calu-6, A549, H460, H661, H2126, H322, H1299, H522, PC9, and H4006), colon (SW620, DLD-1, and HCT-8), pancreatic (MiaPaCa2 and L3.6pl), and prostate (DU145) cancer cell lines and HEK293 cells were obtained from the American Type Culture Collection and cultured in Dulbecco’s modified Eagle’s medium (DMEM) or RPMI-1640 medium. Normal/immortalized T80 cells (J. Liu and R. Bast, MD Anderson Cancer Center) were cultured in Medium 199/MCDB 105. hTERT-immortalized HPNE cells (Channing Der, University of North Carolina) were grown in medium D (mixture of M3 medium and DMEM) containing one volume of M3 Base F culture medium (InCell Corp.), three volumes of glucose-free DMEM, 5.5 mM glucose, 10 ng/mL EGF, and 50 µg/mL gentamycin. All media were supplemented with 10% heat-inactivated fetal bovine serum, 10 U/mL penicillin, and 10 µg/mL streptomycin. SB was synthesized in-house as described previously^{30 (link)} and dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich). Tideglusib, AZD1080, and BIO were purchased from SelleckChem. The Published Kinase Inhibitor Set 1 (PKIS1) of 304 compounds was received from GlaxoSmithKline (GSK). All cell lines were mycoplasma-free, monitored regularly with HEK-blue2 cells and mycoplasma detection kit from invivogen (cat# rep-pt1).