Axioimager m2

Manufactured by Linkam

The Axioimager M2 is a microscope system designed for high-quality imaging and analysis. It features a motorized stage and objective turret, allowing for precise positioning and seamless switching between magnification levels. The system is equipped with advanced optics to capture detailed images of samples.

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Lab products found in correlation

Talos arctica, by Thermo Fisher Scientific (2 mentions) Cms196m, by Linkam (2 mentions) K3 bioquantum, by Thermo Fisher Scientific (2 mentions) Zen 3, by Zeiss (1 mentions) Maps software, by Thermo Fisher Scientific (1 mentions) Zen software, by Adobe (1 mentions)

2 protocols using axioimager m2

Cryo-CLEM Imaging Workflow Protocol

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Cryo-LM was performed on a Zeiss Axioimager M2 equipped with a Linkam CMS196M. Transparent light and fluorescent image stacks (21 slices every 75 µm) we recorded using an EC PlanNeofluor ×10/0.30 Ph1 objective lens on an AxioCam MR R3 at a pixel size at the sample level of 1 µm × 1 µm. Brightfield images were recorded using a LED light source and fluorescent images were recorded using an HXP 120 V light source using a 38 HE filter set. Maximum intensity projections for both image stacks were made using Zeiss ZEN 3.4 software and overlays were made in Adobe Photoshop 2021. Cryo-CLEM was performed on a Talos Arctica (Thermo Fisher Scientific) operated at 200 keV, equipped with a Gatan K3 BioQuantum direct electron detector. Correlations were performed and images were recorded using MAPS software (Thermo Fisher Scientific).

Koning R.I., Vader H., van Nugteren M., Grocutt P.A., Yang W., Renault L.L., Koster A.J., Kamp A.C, & Schwertner M. (2022). Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection. Nature Communications, 13, 2985.

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Cryo-Correlative Light and Electron Microscopy

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CryoLM was performed on a Zeiss Axioimager M2 equipped with a Linkam CMS196M. Transparent light and fluorescent image stacks (21 slices every 75 µm) we recorded using a EC PlanNeofluor 10x/0.30 Ph1 objective lens on an AxioCam MR R3 at a pixel size at the sample level of 1 µm x 1 µm. Brightfield images were recorded using a LED light source and fluorescent images were recorded using a HXP 120 V light source using a 38 HE filter set. Maximum intensity projections for both image stacks were made using Zeiss ZEN software and overlays were made in Adobe Photoshop.
Cryo-CLEM was performed on a Talos Arctica (Thermo Fisher Scientific) operated at 200 keV, equipped with a Gatan K3 BioQuantum direct electron detector. Correlations were performed and images were recorded using MAPS software version 3.0 (Thermo Fisher Scientific).

Koning R.I., Vader H., Nugteren M.v., Grocutt P., Yang W., Renault L., Koster A.J., Kamp A.C.F., & Schwertner M. (2021). Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection.

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