The ESCC cell line TE‐13, ECA109, TE‐1 was purchased from a typical cell culture collection committee of the Chinese Academy of Sciences Library. The cells were cultured in RPMI‐1640 medium (Gibco, CA, USA) supplemented with 10% FBS (Gibco, CA, USA), 100 U/mL penicillin and 100 μg/mL streptomycin at 37°C in a humidified atmosphere of 5% CO2. Cells were routinely examined for Mycoplasma contamination. To induce hypoxic conditions (<1% O2), cells were cultured at 37°C in the same incubator in an AnaeroPack jar with AneroPack‐Anaero (Mitsubishi, Tokyo, Japan) according to the manufacturer's instructions. The hypoxic environment was confirmed by hypoxia inducible factor 1 subunit alpha (HIF‐1α) expression.
Aneropack anaero
Manufactured by Mitsubishi
Sourced in Japan
The AneroPack-Anaero is a laboratory equipment designed for anaerobic sample storage and incubation. It provides an oxygen-free environment for the preservation and cultivation of anaerobic microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using aneropack anaero
1
Hypoxic Culture of ESCC Cell Lines
2
Hypoxia and X-ray Irradiation of OSCC Cell Lines
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Human OSCC cell lines (Te13, Te1 and Eca109) were obtained from American Type Culture Collection (ATCC, USA). All cell lines were cultured in RPMI-1640 medium (Gibco, USA) with 10% foetal bovine serum (FBS; Gibco, USA), 100 U/ ml penicillin and 100 µg/ml streptomycin. Cells were maintained at 37 °C and 5% CO 2 and were routinely examined for Mycoplasma contamination. To induce hypoxia (< 1% O 2 ), cells were cultured at 37℃ in the same incubator in an AnaeroPack jar with AneroPack-Anaero (Mitsubishi, Japan) according to the manufacturer's instructions. The hypoxic environment was con rmed by hypoxia inducible factor 1 subunit alpha (HIF-1α) expression. Cells were irradiated by an RS 2000 Pro X-Ray Bioirradiator (Radsource, USA).
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