Eight-plex iTRAQ reagent kits were acquired from Applied Biosystems; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Monoclonal antibodies against human PIF (18-802-392017) were obtained from GenWay (San Diego, CA, USA) and polyclonal antibodies against GAPDH (ab9485), MYH10 (ab684), tubulin, β5 class I (TUBB5; ab15568) and heat shock protein family D1 (HSPD1; ab46798) were purchased from Abcam (Cambridge, UK). MYH10-specific Stealth Select RNAi™ small interfering (si)RNA (NM_005964.3), Stealth RNAi™ Negative Control siRNA (cat. no. 12935-400) and Lipofectamine 2000 transfection reagent were obtained from Thermo Fisher Scientific, Inc. CytoSelect™ 24-Well Cell Migration and Invasion Assay kits (8 µm, colorimetric format) were purchased from Cell Biolabs (San Diego, CA, USA).
Eight plex itraq reagent kits
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Eight-plex iTRAQ reagent kits are a set of isobaric chemical labels used in quantitative proteomics analysis. These kits enable the simultaneous comparison of up to eight different samples or conditions in a single mass spectrometry experiment.
Automatically generated - may contain errors
Lab products found in correlation
Cytoselect 24 well cell migration and invasion assay kit, by Cell Biolabs (3 mentions)
Protein a g agarose beads, by GE Healthcare (3 mentions)
Lipofectamine max transfection reagent, by Santa Cruz Biotechnology (3 mentions)
Ip lysis buffer, by Beyotime (3 mentions)
Stealth select rnai sirna nm 000567, by Santa Cruz Biotechnology (2 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (2 mentions)
Ab46798, by Abcam (1 mentions)
Ab684, by Abcam (1 mentions)
Ab15568, by Abcam (1 mentions)
Ab9485, by Abcam (1 mentions)
Anti cytokeratin 8, by Abcam (1 mentions)
Anti annexin a2, by Abcam (1 mentions)
4 protocols using eight plex itraq reagent kits
1
Proteomic Analysis of Cell Migration
2
Quantitative Proteomics Analysis Workflow
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Eight-plex iTRAQ reagent kits were acquired from Applied Biosystems (Foster City, CA, USA). Monoclonal antibodies against human CRP were obtained from HyTest (Finland, Turku). Monoclonal antibodies against UGDH, EphB3, ENO2, ANXA2, KRT5, MSH2, SHC1, HSP90B1, MAPK, p-MAPK, ERK, p-ERK and HIF1α were purchased from Abcam (Cambridge, USA). CRP speci c Stealth Select RNAi™ siRNA (NM_000567), Stealth RNAi™ Negative Control siRNA and Lipofectamine Max transfection reagent were obtained from Santa Cruz (California, USA). Small interfering RNA against human EphB3 (ID 146498, ID 146499) were purchased from Invitrogen (Grand Island, NY). IP lysis buffer was purchased from Beyotime (Shanghai, China). Protein A/G agarose beads from GE Healthcare (Little Chalfont, UK). CytoSelect™ 24-Well Cell Migration and Invasion Assay kits (8 µm, colorimetric format) were purchased from Cell Biolabs (San Diego, CA, USA).
3
Quantitative Proteomic Analysis of CRP Regulation
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Eight-plex iTRAQ reagent kits were acquired from App lied Biosystems (Foster City, CA, USA). Monoclonal antibodies against human CRP were obtained from HyTest (Finland, Turku). Monoclonal antibodies against UGDH, EphB3, ENO2, ANXA2, KRT5, MSH2, SHC1, HSP90B1, MAPK, p-MAPK, ERK, p-ERK and HIF1α were purchased from Abcam (Cambridge, USA). CRP specific Stealth Select RNAi™ siRNA (NM_000567), Stealth RNAi™ Negative Control siRNA and Lipofectamine Max transfection reagent were obtained from Santa Cruz (California, USA). Small interfering RNA against human EphB3 (ID 146498, ID 146499) were purchased from Invitrogen (Grand Island, NY). IP lysis buffer was purchased from Beyotime (Shanghai, China). Protein A/G agarose beads from GE Healthcare (Little Chalfont, UK). CytoSelect™ 24-Well Cell Migration and Invasion Assay kits (8 μm, colorimetric format) were purchased from Cell Biolabs (San Diego, CA, USA).
4
Quantitative Proteomics of CRP Regulation
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Eight plex iTRAQ Reagent Kits were acquired from Applied Biosystems (Foster City, CA). Monoclonal antibodies against human CRP were obtained from HyTest (Finland, Turku), and the anti-Cytokeratin 8, anti-Annexin A2, anti-ENO2, and anti-HSP90B1 antibodies were purchased from Abcam (Cambridge, MA). CRPspecific Stealth Select RNAi™ small interfering RNA (siRNA) (NM_000567), Stealth RNAi™ Negative Control siRNA, and Lipofectamine Max transfection reagent were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Small interfering RNAs against human ENO2 (ID121346, ID 121348) and HSP90B1 (ID119655, ID 119656) were purchased from Invitrogen (Grand Island, NY). IP lysis buffer was purchased from Beyotime (Shanghai, China). Protein A/G agarose beads were obtained from GE Healthcare (Little Chalfont, UK).
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