For elemental analysis, serum samples were first diluted to 1:15 (w/w) with an acidified (pH = 2.0) diluent consisting (w/w) of 1% 1-butanol (Merck KGaA, Darmstadt, Germany), 0.1% Triton X-100 (Sigma-Aldrich, Co., St. Louis, USA), and 0.07% HNO3 (Sigma-Aldrich, Co.) in distilled deionized water (18 MOM cm−1) (Merck Millipore, Billerica, Massachusetts, USA). Next, the macronutrient (i.e., Ca, K, Mg, Na, and P), vital microelement (i.e., Co, Cr, Cu, Fe, I, Li, Mn, Se, Si, V, and Zn), and toxic microelement (i.e., As, B, Cd, Hg, Ni, Pb, Sn, and Sr) contents of all samples were determined using a NexION 300D spectrometer (Perkin Elmer, USA). The elemental content of broiler blood samples was assessed within the framework of research project No. 22-16-00070 supported by the Russian Science Foundation.
Nexion 300d spectrometer
Manufactured by PerkinElmer
Sourced in United States
The NexION 300D is an inductively coupled plasma mass spectrometer (ICP-MS) designed for high-performance elemental analysis. It provides accurate and sensitive detection of a wide range of elements in various sample types.
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3 protocols using nexion 300d spectrometer
1
Trace Elements Analysis in Broiler Serum
2
Soil Metal Content Analysis
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To determine total metal content (xx_T), soil samples were acid-digested (HCl:HNO3, 3:1); water-soluble content (xx_W) was obtained from soil/water extract (1:5) [33 ], and bioavailable content (xx_B) was obtained by extraction with 0.05 M EDTA (pH 7), as described by [17 (link)]. All samples were analyzed by ICP-MS in a PerkinElmer NexION® 300D spectrometer (Waltham, MA, USA). The ICP-MS operating conditions included three replicates in each measurement. For calibration, two sets of multi-element standards containing all the analytes of interest were prepared using rhodium as the internal standard. All standards were prepared from ICP single-element (Zn, Cu, Pb, As and Cd) standard solutions (Merck, Darmstadt, Germany) after dilution with 10% HNO3. The accuracy of the method was confirmed by analyzing Certified Reference Material CRM025-050 (Sandy Loam Soil), six replicates. For Zn, Cu, Pb, As and Cd, the average recoveries were 98.4 ± 12% of the CRM.
3
Comprehensive Bentonite Characterization
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X-ray diffraction (XRD) characterization of the three bentonite sites was done as described in Lopez-Fernandez et al. [22] . Composition of the major elements of each sample was determined by X-ray fluorescence spectroscopy (XRF), on pressed pellets made of 1:10 lithium tetraborate dilution. A portion of this pellet was burned at 1000 ºC for 1 hour to calculate the Loss On Ignition (LOI). The instrument used for the XRF measurements was Philips-Magix Pro (PW-2440), Netherland. De Jongh model [29] was used to convert the X-Ray intensities into concentrations (Philips software). Trace elements content of the samples was determined in triplicate by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) using NexION 300d spectrometer (Perkin Elmer) with a precision of ± 5% for an analyte concentration of 10 ppm. Thus, 0.1 g of sample powder was digested with a mixture of HNO3 and HF in a Teflon-lined vessel, evaporated to dryness, and subsequently dissolved in 100 ml of 4 vol. % HNO3.
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