The proteins of transgenic apple plants and GL‐3 were extracted with protein extraction buffer [50 mm Tris‐HCl, pH 8.0, 150 mm NaCl, 2 mm EDTA, 1 mm DTT, 10% glycerol, 1% Triton X‐100, 1 mm phenylmethylsulfonyl fluoride (PMSF) and 1× Halt protease inhibitor cocktail (78427, Thermo Fisher Scientific, Shanghai, China)] and centrifuged at 14 000 g at 4 °C for 10 min. The extracted proteins were used for Western blot analysis with polyclonal MdDREB2A antibody against rabbit, or anti‐SUMO (ab5316, Abcam, Waltham, MA, USA), anti‐Ubiquitin (P4D1, Cell Signaling Technology®, Shanghai, China), anti‐MdRNF4 (rabbit polyclonal antibody, ABclonal Technology, Wuhan, China) or anti‐Actin (AC009, ABclonal Technology, Wuhan, China).
Anti mdrnf4
Manufactured by ABclonal
Sourced in China
Anti‐MdRNF4 is a laboratory reagent used for research purposes. It is a primary antibody that specifically binds to the MdRNF4 protein. MdRNF4 is a protein involved in various cellular processes. The Anti‐MdRNF4 antibody can be used to detect and study the MdRNF4 protein in biological samples.
Automatically generated - may contain errors
2 protocols using anti mdrnf4
1
Protein Extraction and Western Blot Analysis
2
Protein Extraction and Western Blot Analysis of Transgenic Apple Plants
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The proteins of transgenic apple plants and GL-3 were extracted with protein extraction buffer [50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1 mM DTT, 10% glycerol, 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 1 × Halt protease inhibitor cocktail (Fisher Scientific)] and centrifuged at 14,000 g at 4°C for 10 min. The extracted proteins were used for western blot analysis with polyclonal MdDREB2A antibody against rabbit, or anti-SUMO (ab5316, Abcam), anti-Ubiquitin (P4D1, Cell Signaling Technology ® ), anti-MdRNF4 (rabbit polyclonal antibody, ABclonal Technology), or anti-Actin (AC009, ABclonal Technology).
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