Methcathinone

Cathinone, methcathinone, methylone, N-ethylcathinone, buphedrone, mephedrone, 4-methylethcathinone, α-pyrolidinovarophenone (PVP), MDPV, and naphyrone (1 mg/mL), and internal standards MDPV-d8, mephedrone-d3, methylone-d3, and naphyrone-d5 (100 µg/mL) were obtained from Cerilliant (Round Rock, TX, USA). Solid phase extraction (SPE) cation exchange cartridges Strata Drug-X B 60 mg/3 mL were from Phenomenex (Torrance, CA, USA). Glacial acetic acid, acetonitrile, ammonium hydroxide, and formic acid were acquired from Pharmco-Aaper (Shelbyville, KY, USA). Methanol, dichloromethane, and isopropanol were acquired from Fisher Scientific (Pittsburgh, PA, USA). All solvents used in the extraction were high performance liquid chromatography (HPLC) grade and in the chromatographic instrument were liquid chromatography-mass spectrometry (LC-MS) grade. Quantisal buffer was obtained from Immunalysis Corp. (Pomona, CA, USA) and Oral-Eze buffer from Capitol Vial, Inc. (Auburn, AL, USA). Neat drug-free OF was obtained from healthy volunteers by spitting into a Corning® polypropylene 50 mL tube (Fisher Scientific).