Low endotoxin fbs

Manufactured by Atlas Biologicals

Low endotoxin Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. It is processed to reduce endotoxin levels, making it suitable for cell culture applications that require low-endotoxin environments.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Nigericin, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Corning (1 mentions) Poly da dt, by Merck Group (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Nigericin, by InvivoGen (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Acridine orange, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Dmem medium, by Corning (1 mentions) Syto60, by Thermo Fisher Scientific (1 mentions) Ca 074me, by Enzo Life Sciences (1 mentions) Pepstain a, by Merck Group (1 mentions) Cellmask plasma membrane stain, by Thermo Fisher Scientific (1 mentions) Calcein am, by Thermo Fisher Scientific (1 mentions) Lysotracker, by Thermo Fisher Scientific (1 mentions)

2 protocols using low endotoxin fbs

Lipofectamine 2000 Transfection Protocol

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Lipofectamine 2000 was obtained from Invitrogen. Poly(dAdT) and nigericin were obtained from Sigma-Aldrich. Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Cellgro, and low endotoxin FBS was obtained from Atlas Biologicals. The ELISA kit for mouse IL-1β was from R&D Systems.

Tzeng T.C., Schattgen S., Monks B., Wang D., Cerny A., Latz E., Fitzgerald K, & Golenbock D.T. (2016). A fluorescent reporter mouse for inflammasome assembly demonstrates an important role for cell bound and free ASC specks during in vivo infection. Cell reports, 16(2), 571-582.

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Reagent Inventory for Cell Biology Experiments

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Lipofectamine 2000 was from Invitrogen (Carlsbad, CA). Poly (dAdT), Cytochalasin D and Pepstain A, acridine orange and bromodeoxyuridine were from Sigma-Aldrich. Bafilomycin A1 and giemsa stain were from Fluka Analytical. Alexa Fluor 546-conjugated dextran, Lysotracker, Cell Mask Plasma membrane stain, DAPI, SYTO 60, Calcein AM and propidium iodide were from Molecular Probes/Invitrogen (Carlsbad, CA). Nigericin was from Invivogen; CA-074Me was from Enzo LifeSciences; Cat L inhibitor was from Calbiochem; uricase (Elitek) was from Sanofi-Aventis. DMEM medium was from Cellgro (Manassas, VA) and low endotoxin FBS was from Atlas Biologicals (Fort Collins, CO). CpG DNA oligodeoxynucleotide 1826 and CpG-Alexa 647 and AT2-A647 were obtained from IDT (Coralville, IA). Pf 3D7 genomic DNA was purified as described (Parroche et al., 2007 (link)). Pf HB3_B2, Pf T9/94 and Pf Dd2 genomic DNA were obtained from the malaria research and reference resource center at NIAID.

Kalantari P., DeOliveira R.B., Chan J., Corbett Y., Rathinam V., Stutz A., Latz E., Gazzinelli R.T., Golenbock D.T, & Fitzgerald K.A. (2014). Dual engagement of the NLRP3 and AIM2 inflammasomes by plasmodial-derived hemozoin and DNA during malaria. Cell reports, 6(1), 196-210.

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