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1 stearoyl 2 arachidonoyl sn glycero 3 phosphocholine

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1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine is a phospholipid compound. It is a key structural component of cell membranes.

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Lab products found in correlation

1 palmitoyl 2 linoleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions) Mini extruder, by Avanti Polar Lipids (1 mentions) 1 2 distearoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 stearoyl 2 arachidonoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) 1 stearoyl 2 arachidonoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (1 mentions) 11 oh thc, by Cerilliant (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) 1 stearoyl 2 hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Methanol, by Honeywell (1 mentions) Lc ms grade water, by Honeywell (1 mentions) Hplc grade solvents, by Avantor (1 mentions) 1 heptadecanoyl 2hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 docosahexaenoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 azelaoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 stearoyl 2 linoleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 arachidonoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 5 oxo valeroyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)

4 protocols using 1 stearoyl 2 arachidonoyl sn glycero 3 phosphocholine

1

Radiolysis of Lipid Liposomes

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HOCH 2 CH 2 SH and 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (POPC) were purchased from Aldrich. 1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) and 1-stearoyl-2-arachidonoyl-snglycero-3-phosphocholine (SAPC) were purchased from Avanti Polar Lipids (Alabaster, AL) (see Fig 1B). All solvents were HPLC-grade and the utilized water was glass-distilled. Radiolysis was performed at room temperature (22 ± 2 °C) on 1 mL samples using a 60 Co Gammacell. For the construction of 100 nm liposomes the Mini Extruder was used from Avanti Polar Lipids. The composition of lipids during the experiments was monitored by gas chromatography using the GC 7890Β from Agilent equipped with a flame ionization detector. The column used was the DB-23 capillary column (60m × 0.25mm). The heating was carried out at a temperature of 165 °C for 3 minutes followed by an increase of 1 o C/min up to 195 °C. The temperature remained at 195 °C for 40 minutes and then it was followed by an increase of 10 °C /min up to 240 °C for 10 minutes.
Tremi I., Anagnostopoulos D., Spyratou E., Gkeka P., Georgakilas A.G., Chatgilialoglu C, & Cournia Z. (2018). Effect of 5-trans Isomer of Arachidonic Acid on Model Liposomal Membranes Studied by a Combined Simulation and Experimental Approach. The Journal of membrane biology, 251(3).
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2

Lipid Biomarker Quantification Protocol

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1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC, PC(18:0_18:0)), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC, PC(18:0_20:4)), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine (SAPE, PE(18:0_20:4)) and 1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-L-serine (SAPS, PS(18:0_20:4)) were from Avanti® Polar Lipids, Inc (Alabaster, AL, USA). 1,2-dimyristoyl-sn-glycero3-PS (DMPS, PS(14:0_14:0)) and 1,2-dimyristoyl-sn-glycero-3-PE (DMPE, PE(14:0_14:0)) were biotinylated and used as internal standards to assess PS/PE externalization by mass spectrometry, using N-Hydroxysuccinimide (NHS)-biotin as described [15 , 29 (link)].
Rosas M., Slatter D.A., Obaji S.G., Webber J.P., Alvarez-Jarreta J., Thomas C.P., Aldrovandi M., Tyrrell V.J., Jenkins P.V., O’Donnell V.B, & Collins P.W. (2020). The procoagulant activity of tissue factor expressed on fibroblasts is increased by tissue factor-negative extracellular vesicles. PLoS ONE, 15(10), e0240189.
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3

Quantification of Endocannabinoid Metabolites

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Authentic THC, 11-OH-THC, and 11-COOH-THC standards and their corresponding 2H-containing derivatives were obtained from Cerilliant (Round Rock, TX). THC for animal administration was purchased from Sigma-Aldrich (St. Louis, MO). LC/MS-grade water and methanol were from Honeywell (Muskegon, MI). LC/MS-grade acetonitrile (ACN), isopropanol, and acetone were from Sigma-Aldrich. Formic acid (FA) was from Thermo Fisher (Houston, TX); 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine (18:0 lysophosphatidylcholine, 18:0 LPC) and 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (18:0–20:4 phosphatidylcholine, 18:0–20:4 PC) were from Avanti Polar Lipids (Alabaster, Alabama).
Vozella V., Zibardi C., Ahmed F, & Piomelli D. (2019). Fast and Sensitive Quantification of Δ9-Tetrahydrocannabinol and Its Main Oxidative Metabolites by Liquid Chromatography/Tandem Mass Spectrometry. Cannabis and Cannabinoid Research, 4(2), 110-123.
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4

Phospholipid Standards for Lipidomics

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HPLC grade solvents were obtained from VWR International (Mississauga, Ontario, Canada). The standard phospholipids; 1,2-dinonanoyl-sn-glycero-3-phosphocholine (09:0 PC), 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (17:0 LPC), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC), 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PAPC), 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SLPC), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDHPC), 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PAzPC) and 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PONPC) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), 1-palmitoyl-2-(5’-keto-6’-octenedioyl)-sn-glycero-3-phosphocholine (KOdiA-PC) and 1-palmitoyl-2(4’-keto-dodec-3’-ene-dioyl)-sn-glycero-3-phosphocholine (KDdiA-PC) were purchased from Cayman Chemicals (Ann Arbor, Michigan, USA). All other chemicals were purchased from Sigma Aldrich (St. Louis, Missouri, USA).
Solati Z., Edel A.L., Shang Y., O K, & Ravandi A. (2018). Oxidized phosphatidylcholines are produced in renal ischemia reperfusion injury. PLoS ONE, 13(4), e0195172.
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