On Day 28 (for evaluating the effects of Huashi Pill) post Huashi Pill administration, the plasma was obtained from the blood treated with 11% trisodium citrate (Sangon Biotech Co., Ltd., Shanghai, China) using centrifugation. The aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TBIL) in plasma were evaluated with commercial kits according to the manufacturers’ instructions and standard methods [15 (link)].
Trisodium citrate
Manufactured by Sangon
Sourced in China
Trisodium citrate is a chemical compound with the formula Na3C6H5O7. It is a white crystalline solid that is commonly used as a pH buffer and sequestering agent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using trisodium citrate
1
Evaluating Huashi Pill Liver Effects
2
Blood Lipid Profiling Protocol
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Venous blood samples of 5 ml were drawn after at least 12 h of fasting. A total of 2 ml of the sample was collected into a glass tube and used to determine serum lipid levels. The remaining 3 ml was transferred to tubes with anticoagulants (4.80 g/l citric acid, 14.70 g/l glucose and 13.20 g/l trisodium citrate; Shanghai Sangon Biological Engineering Technology & Services Co.) and used to extract DNA. Measurements of serum TC, TG, HDL-C and LDL-C levels in the samples were performed using enzymatic methods with a Ransod autoanalyzer (Randox Laboratories Ltd., Crumlin, UK and Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan). Serum ApoA1 and ApoB levels were detected using the immunoturbidimetric immunoassay using a commercial kit (Randox Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University (27 (link)–30 (link)).
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