Mart1 peptide

Manufactured by ProImmune

Sourced in United Kingdom

MART1 peptide is a synthetic peptide used in laboratory research. It is derived from the Melanoma-associated antigen recognized by T cells 1 (MART1) protein. The MART1 peptide serves as a tool for studying immune responses and cancer research.

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Lab products found in correlation

Infinite 200 microplate reader, by Tecan (1 mentions) Human il 2 duoset elisa, by R&D Systems (1 mentions) Celltracker blue, by Thermo Fisher Scientific (1 mentions) Evos fl digital inverted microscope, by Thermo Fisher Scientific (1 mentions)

2 protocols using mart1 peptide

Measuring IL-2 Secretion in T-Cell Activation

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For activation by SEE: Raji B cells were resuspended at 10⁶ cells/ml, and 50 μl were seeded in 96-well flat-bottom plates. Jurkat wt or IRAP ko cells were resuspended at 10⁶ cells/ml, and 100 μl were added to the Raji B cells. In total, 50 μl SEE (Toxin Technology) was added at the indicated final concentrations, and supernatants were harvested after 6 h incubation at 37 °C, 5% CO₂.
For activation by MART1 peptide: 15 × 10³ Daju-A2 cells per well were cultured in a 96-well flat-bottom plate overnight. The day after, MART1 peptide (ProImmune) was added at the indicated final concentrations, as well as 200 × 10³ Jurkat wt or IRAP ko cells expressing TCR-MelanA2. Supernatants were harvested after overnight incubation at 37 °C, 5% CO₂.
IL-2 was measured by ELISA with the kit Human IL-2 DuoSet ELISA (R&D Systems) on a Tecan Infinite 200 microplate reader.

Evnouchidou I., Chappert P., Benadda S., Zucchetti A., Weimershaus M., Bens M., Caillens V., Koumantou D., Lotersztajn S., van Endert P., Davoust J., Guermonprez P., Hivroz C., Gross D.A, & Saveanu L. (2020). IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses. Nature Communications, 11, 2779.

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Evaluating MART1-Specific T Cell Cytotoxicity

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CD8⁺ T cells obtained from a healthy HLA-A*02-01 donor were transduced using a lentiviral vector encoding a MART1-specific TCR, which was a generous gift donated by Richard Koya (20 (link)). Primary PC cells isolated as previously described (16 (link),17 (link)), were evaluated for HLA A2 expression using flow cytometry with a commercially validated antibody (Biolegend) and a representative HLA A2-positive line was chosen. 2.5×10⁴ PC cells were seeded in 96 well plates and allowed to adhere overnight in growth medium. Transgenic T cells were added at the indicated effector:target T cell ratio in the presence and absence of the recombinant MART1 peptide (12.5 µM, ProImmune Ltd., Oxford, United Kingdom). Images were collected on an EVOS FL digital inverted microscope (Life Technologies, Carlsbad, CA) and processed with ImageJ (NIH). The CellTracker™ Blue (CMAC) dye was used to label T cells and co-cultures were performed in the presence of SYTOX® Green for live cell imaging (Thermo Fisher Scientific).

Delitto D., Delitto A.E., DiVita B.B., Pham K., Han S., Hartlage E.R., Newby B.N., Gerber M.H., Behrns K.E., Moldawer L.L., Thomas R.M., George TJ J.r., Brusko T.M., Mathews C.E., Liu C., Trevino J.G., Hughes S.J, & Wallet S.M. (2016). Human Pancreatic Cancer Cells Induce a MyD88-Dependent Stromal Response To Promote a Tumor-Tolerant Immune Microenvironment. Cancer research, 77(3), 672-683.

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