Skov3 cells

Cisplatin-resistant SKOV3/DDP and parental SKOV3 cells were purchased from China Center for Type Culture Collection and preserved at the Key Laboratory of Molecular Center of Jiangxi Province. The cells were routinely cultured in RPMI-1640 medium containing fetal bovine serum (FBS; 10%), penicillin/streptomycin (100 U/ml) in a 5% humidified CO₂ atmosphere at 37°C. Cisplatin (0.3 µg/ml) was added to the SKOV3/DDP culture media to maintain its acquired resistance.

Human THP-1 monocytes, SKOV3 cells, and A2780 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). THP-1 cells and A2780 cells were maintained in RPMI-1640, and SKOV3 cells were cultured with McCoy’s 5A medium. These media are all from Hyclone (Logan, UT, USA). The cell lines were cultured in medium supplemented with 10% FBS (Gibco, Rockville, MD, USA), 100 μg/mL streptomycin, and 100 U/mL penicillin (Gibco, Rockville, MD, USA) at 37°C in a humidified atmosphere with 5% CO₂. THP-1 cells were seeded at 1 × 10⁵ cells per well in 6-well plates and incubated with PMA at 50 ng/mL for 12 h to induce M0 macrophages. Then, the M0 macrophages were induced to TAMs with 50% (v/v) SKOV3 CM for another 48 h. In parallel, M0 macrophages were directed to the M1 phenotype with LPS at a final concentration of 20 ng/mL or were polarized to M2 with 20 ng/mL IL-4.

Human ovarian cancer cells (SKOV3 cells) and human ovarian epithelial cells (IOSE80 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences. RhApoA-I and rhApoE were prepared and stored in our laboratory. Sal was purchased from Selleck Co., Ltd. (China). Rabbit antibodies against c-Myc, Sox-2, Nanog, Oct-4, E-cadherin, vimentin, N-cadherin, β-catenin, and cleaved caspase-3 were obtained from Cell Signaling Technology (USA). Rabbit antibodies against LRP-1, Bax, and Bcl-2 were purchased from Abcam (Cambridge, USA). The Annexin V-FITC/PI apoptosis detection kit and PE-conjugated anti-human CD133 antibody were purchased from BD Biosciences (USA). The biotin-conjugated anti-human CD133 antibody was purchased from Miltenyi Biotec (Germany). The CELLection Biotin Binder Kit was purchased from Invitrogen (USA). The basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) were bought from Peprotech (USA). The B27 supplement was obtained from Gibco (USA). The DMEM and DMEM/F12 medium, RIPA 1640, and Fetal Bovine Serum (FBS) were purchased from Biological Industries (Israel). Lecithin and coumarin 6 (C6) were purchased from Sigma-Aldrich (USA). Protease inhibitors were obtained from Thermo Fisher Scientific (USA). Horseradish Peroxidase-conjugated secondary antibody was bought from Absin Bioscience Co., Ltd. (China).

Human ovarian epithelial adenocarcinoma SKOV3 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and endometrioid-type ovarian epithelial carcinoma 59M cells were purchased from the European Collection of Authenticated Cell Cultures; Public Health England (Salisbury, UK). SKOV3 cells were cultured in McCoy's 5A medium (Thermo Fisher Scientific, Inc., Waltham, MA) and 59M cells in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Lonza Group, Basel, Switzerland), 2 mM L-glutamine, 100 µg/ml streptomycin and 100 units/ml penicillin. Cells were incubated at 37°C in a humidified atmosphere containing 5% CO₂.