V plex human mcp 1 kit

Cytokines and chemokines (IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-α, and MCP-1) in plasma and supernatants were measured using the V-PLEX Proinflammatory Panel 1 Human Kit and V-PLEX Human MCP-1 kit (Meso Scale Diagnostics) as per the manufacturers’ instructions. For the data points that were below the detection range, half of the lower limit of detection was used as a value in the analyses.

Inflammatory markers were measured at baseline and month 12 as previously described (22 (link), 36 (link)). Serum C-reactive protein (CRP) was measured using an Immulite (Siemens Healthcare, Munich, Germany) high-sensitivity CRP kit as per manufacturer’s instructions. Peripheral blood mononuclear cells (PBMCs) were isolated as previously described (22 (link), 37 (link)). Cytokines and chemokines (IL-1β, IL-6, IL-8, TNF-α, and MCP-1) in plasma and supernatants harvested from lipopolysaccharide (LPS)-stimulated PBMCs were measured using the V-PLEX Proinflammatory Panel 1 Human Kit and V-PLEX Human MCP-1 kit (Meso Scale Diagnostics, LLC, Rockville, MD) as per manufacturer’s instructions. Plasma 8-isoprostane and serum total antioxidant capacity (TAC) concentrations were measured using Cayman’s 8-isoprostane kit and antioxidant assay kit, respectively (Cayman Chemicals) as per manufacturer’s instructions (intra- and inter-assay CVs were <10% for both assays). Each assay was performed in duplicate. The number and activation of circulating monocytes in PBMCs were quantified using flow cytometric analysis as previously described (38 (link)).