The following primary antibodies were used for IHC or western blot analyses: mouse anti-GPR56 (H11) (1:200)40 (link) and rabbit anti-GPR56 (199) (1:200)19 (link), rabbit anti-MBP (Millipore; Cat #AB980, 1:200), rat anti-MBP (Abcam, Cat# ab7349), mouse anti-O4 (Millipore; Cat #MAB345, 1:400), rabbit anti-NG2 (Millipore; Cat #AB5320, 1:200), goat anti-Sox2 (Santa Cruz; Cat #sc-17320, 1:400), rabbit anti-Olig2 (kind gift from Charles Stiles, 1:10,000), rat anti-PDGFR (BD Bioscience; Cat #558774, 1:500), rabbit anti-PDGFR (Cell Signaling Technologies; Cat #3164S, 1:500) and rat anti-Ki67 (Affymetrix eBioscience; Cat #14-5698-80, 1:100), rat anti-BrdU (Accurate Chemical and Scientific Corporation; Cat #OBT0030S, 1:100), rabbit anit-PLP (Abcam, Cat #ab28486, 1:1000), mouse anti-RhoA (Cytoskeleton, Cat# ARH03-A, 1:500), mouse anti-CDK2 (Santa Cruz; Cat #sc-6248, 1:1000), mouse anti-β-actin (Sigma, Cat #A5044, 1:5000), and mouse anti-Ki67 (BD Bioscience; Cat #550609, 1:100). Secondary antibodies were goat anti-mouse or anti-rat conjugated with either Alexa 488 (Life Technologies, 1:1000) or Alexa 546 (Life Technologies,1:1000) and goat anti-rabbit conjugated with Alexa 546 or 555 (Life Technologies, 1:1000), goat anti mouse or rabbit IgG-HRP (Sigma, Cat# A4416 or A6154, 1:3000).
Rabbit anti pdgfr
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-PDGFR is a primary antibody that specifically recognizes the platelet-derived growth factor receptor (PDGFR) protein. It is designed for use in various immunodetection techniques to identify and study the PDGFR protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti sox2, by Santa Cruz Biotechnology (1 mentions)
Alexa 546, by Thermo Fisher Scientific (1 mentions)
Mouse anti cdk2, by Santa Cruz Biotechnology (1 mentions)
Mouse anti rhoa, by Cytoskeleton (1 mentions)
Rat anti mbp, by Abcam (1 mentions)
Rabbit anti ng2, by Merck Group (1 mentions)
Rabbit anti mbp, by Merck Group (1 mentions)
Mouse anti o4, by Merck Group (1 mentions)
Rat anti ki67, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse or rabbit igg hrp, by Merck Group (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Mouse anti ki67, by BD (1 mentions)
Rabbit anti erk, by Cell Signaling Technology (1 mentions)
Iblot2 system, by Thermo Fisher Scientific (1 mentions)
Rabbit anti egfr, by Cell Signaling Technology (1 mentions)
Rabbit anti p p70s6k, by Cell Signaling Technology (1 mentions)
Rabbit anti p erk, by Cell Signaling Technology (1 mentions)
Mouse anti e cadherin, by Agilent Technologies (1 mentions)
Mouse anti n cadherin, by Agilent Technologies (1 mentions)
3 protocols using rabbit anti pdgfr
1
Comprehensive Antibody Panel for Cell Identification
2
Protein Expression Analysis Protocol
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Cells were lysed in RIPA buffer (Merck Millipore, Vimodrone, MI, Italy) containing PMSF (Sigma-Aldrich, Saint Louis, Missouri, USA), sodium orthovanadate (Sigma-Aldrich), and protease inhibitor cocktail (Calbiochem, San Diego, CA, USA), sonicated and centrifuged 15 min at 14,000 rpm at 4 °C. Equal amounts of protein were separated on Bolt® Bis-Tris Plus gels, 4–12% precast polyacrylamide gels (Life Technologies, Monza, Italy). Fractionated proteins were transferred to a PVDF membrane using the iBlot 2 System (Life Technologies). Following 1 h blocking with Odyssey blocking buffer (Dasit Science, Cornaredo, MI, Italy), membrane was probed overnight at 4 °C with mouse anti -N-Cadherin and mouse anti-E-Cadherin (1:1000, DAKO, Glostrup, Denmark); rabbit anti-EGFR, (1:500 Cell Signalling Technology, Danvers, MA, USA); rabbit anti-PDGFR, rabbit anti p-p70S6k, rabbit anti-pERK, rabbit anti-ERK (1:1000, Cell Signalling Technology, Danvers, MA, USA), and 1 h at room temperature with goat anti-rabbit IgG Alexa Flour 750 antibody (Invitrogen, Monza, Italy). Membrane was visualised by the Odyssey Infra-red Imaging System (LI-COR® Bioscience, Lincoln, Nebraska USA). Anti α-tubulin antibody (1:2000, Sigma-Aldrich) was used to assess equal amount of protein loaded in each lane.
3
Western Blot Analysis of Signaling Proteins
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Protein lysates from each cell sample were collected, quantified with the Bradford assay, and boiled with loading buffer for 10 min. A total of 10 μg of protein was used to run in 10% or 12% SDS-PAGE gel, and transferred electrophoretically to a PVDF membrane using a standard method. After blocking with skimmed milk, membrane was incubated with rabbit anti-PTPN2 (1 : 1000), rabbit anti-PDGFR (1 : 2000), p-PDGFR (1 : 1000), Akt (1 : 3000), p-Akt (1 : 2000), Erk (1 : 3000), p-Erk (1 : 3000), or GAPDH (1 : 5000) purchased from Cell Signaling Technology (Denvers, MA, USA), then incubated with secondary rabbit antibodies (1 : 10000, Cell Signaling Technology), and visualized using the enhanced chemical luminescence method.
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