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Serum gel monovette

Manufactured by Sarstedt
Sourced in Germany

Serum-gel monovettes are blood collection tubes used for the separation and storage of serum samples. They contain an inert gel that forms a barrier between the cellular components and the serum after centrifugation, facilitating the safe transport and analysis of serum samples.

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6 protocols using serum gel monovette

1

Salivary and Blood Cortisol Sampling

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Salivary samples were collected by means of Salivette swabs (Sarstedt, Nümbrecht, Germany). The sampling consisted in the intra-orally moistening of a cotton roll for 1 min. prior placement into a salivette swab. Before analysis, the samples were centrifuged at 3000 rpm for 5 min. to produce a clear supernatant of low viscosity. 50 μl were removed for cortisol analysis using a commercially available immunoassay with chemiluminescence detection. The lower detection limit of this assay is 0.43 nmol/l. Intra- and inter-assay coefficients of variation were below 8% for low (3 nmol/l) and high (25 nmol/l) cortisol levels, respectively. Concerning the collection of blood and ACTH samples, blood was extracted by means of an intravenous cannular after 45 min. of rest at the laboratory. The blood samples resided into Serum-Gel-Monovette® (Sarstedt, Nümbrecht, Germany) tubes. Directly after collection, the samples were centrifuged for 10 min at 4 °C and 3000 rpm. Following aliquoting, the samples were stored at −80 °C and at −20 °C freezer before being assayed.
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2

Evaluating Biomarkers in ARDS Patients

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Serum obtained from ARDS patients was collected at both hospitals in a Serum-Gel Monovette (S-Monovette, Sarstedt AG & Co. KG, Nuembrecht, Germany) within 24 h after ICU admission. Samples were centrifuged at 2500 G for 10 min at room temperature. Immediately afterwards, the samples were stored at −80 °C until further processing. Levels of lung epithelial injury markers SP-D and RAGE, endothelial injury marker Ang-2 as well as pro-inflammatory cytokines IL-6 and IL-8 were analyzed by multiplex immunoassay (Luminex Assay, Bio-Techne, Minneapolis, MN, USA).
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3

Serum Ang-2 Quantification by ELISA

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The patients’ blood was collected prior to therapy in serum-gel monovettes (Sarstedt, Germany) and was prepared as previously described (Ding et al. 2022 (link)). All serum samples were stored at − 80 °C and thawed on ice right before measurement. Serum Ang-2 was measured with the Human Angiopoietin-2 Quantikine ELISA set (R&D Systems, Minneapolis, Minnesota, USA, Catalog No. DANG-20). Serum samples were diluted 1:5. The procedure was performed according to protocol provided by the manufacturers.
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4

Venous Blood Sampling and Biomarker Analysis

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Venous blood sampling was performed using ethylenediaminetetraacetic acid (EDTA) and serum-gel monovettes (Sarstedt, Germany). Subsequently, the following parameters were analyzed in the central laboratory of the BG University Hospital Bergmannsheil Bochum immediately after sampling: glycated hemoglobin (HbA1c), total cholesterol, HDL-cholesterol, LDL-cholesterol, and thyroid-stimulating hormone (TSH). Conversely, Hcy was analyzed at the Immunological Laboratory of the University Hospital of Pediatrics and Adolescent Medicine, St. Josef-Hospital, Bochum, where the procedure has been established. Thus, within a time lag of 1 week to the first appointment, fasting venous blood samples were collected in EDTA monovettes (Sarstedt, Germany) and were promptly put on ice. Plasma was obtained by centrifugation (4000x g, 10 min, 4 °C) and stored at − 80 °C until further analysis of Hcy. Upon completion of the entire sample collection, an Enzyme-linked Immunosorbent Assay (ELISA) Kit (EKX-UAP3O8–96, Nordic BioSite, Sweden) was used to measure homocysteine levels in plasma in a total of three batches.
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5

Immunoglobulin Profiles in Cystic Fibrosis

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This is a retrospective chart review as part of the CF registry data from the period 1st January 1984 - 1st June 2016. The ethical approval for the study was approved by the Research Ethical Committee of King Faisal Specialist Hospital and Research Centre in Saudi Arabia with reference number ORA/1179/37. Immunoglobulins A, E, G and M were quantified in all confirmed CF the patients for all age groups at baseline (stage 1) and two follow ups at stages 2 and 3 at time intervals shown in Table 3. Serum from patient blood samples were collected following previously published procedure (Shead et al., 2010 (link)). Briefly, peripheral blood collected from patients were added into serum-gel monovettes (Sarstedt, UK) and mixed well, then allowed to clot. Serum was frozen at −80 °C within 2 hours of separation or used for enzyme linked immunosorbent assay (ELISA). The serum immunoglobulins levels were all measured using ELISA kit.
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6

Exercise Effect on Asprosin Levels

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Fifteen male healthy participants, age = 27.5 ± 3.1 years, weight = 78 ± 7.7 kg, height = 1.81 ± 0.05 m, BMI = 23.9 ± 1.9 kg/m2 were recruited by German Sport University Cologne, Cologne, Germany to study the effect of exercise on the serum asprosin level (Supplementary Table S7). Inclusion criteria were age from 20 to 35 years and physical fitness. Exclusion criteria were musculoskeletal disorder, acute and chronic injuries, trauma or surgery of lower extremities’ joints. Subjects were asked not to exercise 24 h prior to the study and to come to the institute either by car or public transportation. The subjects remained seated for 30 min before the first blood sampling (t0). Further blood samples were drawn immediately afterwards (t1), and then 30 min (t2), 60 min (t3), and 120 min (t4) after running. Blood (5.0–7.5 ml) was collected by venipuncture using serum-gel monovettes1 (Sarstedt, Nümbrecht, Germany). After blood coagulation (30 min, RT), serum was isolated by centrifugation (10 min, 3000 rpm) and stored at − 80 °C.
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