4 hydroxyestradiol

Estrone(E1), 17β-estradiol(E2), estriol(E3), 2-hydroxyestrone(2-OHE1), 2-hydroxyestradiol(2-OHE2), 2-methoxyestrone(2-MeOE1), 2-methoxyestradiol(2-MeOE2), 4-hydroxyestrone(4-OHE1), 4-hydroxyestradiol(4-OHE2), 4-methoxyestrone(4-MeOE1), 4-methoxyestradiol(4-MeOE2), 16α-hydroxyestrone(16α-OHE1), d5-E2, dansyl chloride, Vitamin C, naloxone, naloxone-glucuronide, D-Saccharic acid 1,4-lactone monohydrate and UGPGA were supplied by Sigma-Aldrich (St. Louis, USA) and Steraloids (Rhode, USA).
Tamoxifen was obtained from Aladdin (Shanghai, China). Alamethicin was obtained from J&K Scientific. TRIzol® and PrimeScript RT Reagent Kit were purchased from TaKaRa Bio-technology Co., Ltd. (Beijing, China). Human liver microsomes (HLMs) were acquired from Rild Liver Disease Research Co., Ltd (Shanghai). All analytical solvents were of liquid chromatography (LC) - grade and were obtained from Sigma-Aldrich (St. Louis, USA) or Merck (Darmstadt, Germany).

7,12-Dimethylbenz[α]anthracene (DMBA), mitomycin C, mithramycin A, 4-hydroxyestradiol, 2-hydroxyestradiol, and charcoal-stripped FBS were purchased from Sigma (St. Louis, MO, USA). 2,2′,4,6′-Tetramethoxystilbene (TMS) was kindly provided by Dr. Sanghee Kim (Seoul National University, Seoul, Korea). Rabbit polyclonal antibody for E-cadherin was purchased from Millipore (Bedford, MA, USA). M-MLV reverse transcriptase and RNase inhibitor were purchased from Promega (Madison, WI, USA). Ex Taq Polymerase was obtained from TaKaRa Bio (Shiga, Japan). SYBR green was purchased from QIAGEN (Hilden, Germany). Rabbit polyclonal antibodies for CYP1B1, Sp1, β-catenin, E-cadherin, cyclin D1, vimentin, SNAI1, and GAPDH; mouse monoclonal antibody for ZEB2 and c-Myc; Texas Red-conjugated goat anti-rabbit IgG; and UltraCruz^TM Mounting Medium were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HRP-conjugated goat anti-rabbit IgG and DyLight^® 594-conjugated goat anti-mouse were obtained from Bethyl (Montgomery, TX, USA) and mouse monoclonal antibody for PCNA was purchased from Cell Signaling Technology (Beverly, MA, USA). Other chemicals and reagents were of the highest quality commercially available.