Fitc fluorescein isothiocyanate

Manufactured by Merck Group

Sourced in United States

FITC (fluorescein isothiocyanate) is a fluorescent dye commonly used in various laboratory applications. It has an excitation wavelength of approximately 494 nm and an emission wavelength of around 521 nm, producing a green fluorescent signal. FITC is a reactive chemical compound that can be conjugated to a variety of biomolecules, including proteins, antibodies, and nucleic acids, to enable their detection and visualization in experimental setups.

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Lab products found in correlation

Fluoromont aqueous mounting medium, by Merck Group (1 mentions) Axio observer 7, by Zeiss (1 mentions) Il 27, by Thermo Fisher Scientific (1 mentions) Dspe peg 2000 amine, by Avanti Polar Lipids (1 mentions)

2 protocols using fitc fluorescein isothiocyanate

Fluorescent Labeling of Parasites

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Parasites were incubated at 37°C for 1 hr on glass coverslips as previously described (de Miguel et al., 2010 (link)). Parasites attached to coverslips were fixed in 4% paraformaldehyde at room temperature for 20 min and labeled with WGA lectin from Triticum vulgaris conjugated with FITC(fluorescein isothiocyanate) (Sigma). To this end, parasites were incubated 1:100 WGA/PBS dilution at 37°C during 1 hr, washed three times with PBS solution and mounted using Fluoromont Aqueous Mounting Medium (Sigma). Fluorescent parasites were visualized using a Zeiss Axio Observer 7 (Zeiss) microscope.

Salas N., Blasco Pedreros M., dos Santos Melo T., Maguire V.G., Sha J., Wohlschlegel J.A., Pereira-Neves A, & de Miguel N. (2023). Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication. eLife, 12, e86067.

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Synthesis and Characterization of ART-1-Lipopeptide

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IL-27 was purchased from PeproTech, USA. DOPC (1, 2-Dioleoyl-sn-glycero-3-phosphocholine), DOPE (1, 2-Dioleoyl-sn-glycero-3-phosphoethanolamine), cholesterol and FITC (fluorescein isothiocyanate) were obtained from Sigma-Aldrich, USA. DSPE-PEG (2000) amine (1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N- [amino (polyethylene glycol)-2000] (ammonium salt)) was purchased from Avanti Polar Lipids, USA. Cyanine 7 azide (Cy7) was purchased from Lumiprobe, USA. ART-1-lipopeptide (CRNADKFPC-NH-C₁₈H₃₇) was customsynthesized by GenScript/Lifetein, USA. The structure of ART-1-lipopeptide is shown in Fig. S1. The peptide in both the lipopeptide and liposomal formulation used in this study was found to be in cyclic form (C-C disulfide 1–9) as assessed by LC- MS spectra of ART-1-lipopeptide: m/z calculated 1301.74 for CRNADKFPC-NH-C₁₈H₃₇, found 651.88 ([M+H]+/2) and 434.92 ([M+H]+/3) (protonated molecular ions of the formula (M+H)+ in positive ionization mode).

Meka R.R., Venkatesha S.H, & Moudgil K.D. (2018). Peptide-directed Liposomal Delivery Improves the Therapeutic Index of an Immunomodulatory Cytokine in Controlling Autoimmune Arthritis. Journal of controlled release : official journal of the Controlled Release Society, 286, 279-288.

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