Pcdna3.1 anxa2

The normal immortalized human pancreatic epithelial cell line (HPDE6C7) and four human pancreatic cancer cell lines (CFPAC‐1, BXPC3, L3.6pl and Panc‐1) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All the relevant cells were cultured in DMEM (Sigma‐Aldrich, St. Louis) supplemented with 10% fetal bovine serum (FBS; HyClone, Logan, USA) and kept in a humidified incubator with 5% carbon dioxide at 37°C. The pcDNA3.1‐SNHG14 and pcDNA3.1‐ANXA2 (vector with SNHG4 or ANXA2 overexpression) and empty vectors were from GenePharma (Shanghai, China). The miR‐613 mimic and its negative control (NC), and SNHG14 small interfering RNA (siRNA) (si‐SNHG14) and scrambled siRNA for SNHG14 (si‐NC) were from RiboBio (Guangzhou, China). For cell transfections, cells were grown on 6‐well plates until 60% confluence, and then cells were transfected by miRNA, siRNA, or plasmid using Lipofectamine 2000 reagent (Invitrogen, Carlsbad).

pcDNA3.1-LINC00659, pcDNA3.1-ANXA2, pcDNA3.1, sh-LINC00659, miR-342-3p mimic, miR-342-3p inhibitor and their negative controls were obtained from Shanghai GenePharma Co. Ltd (Shanghai, China). All transfection was carried out using lipfectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol. The exosomes (from CAFs transfected with pcDNA3.1-LINC00659 or pcDNA3.1) incubated with CRC cells were named as pc-LINC00659-exo group or pc-exo group. The exosomes (from CAFs transfected with sh-LINC00659 or sh-NC) incubated with CRC cells were named as sh-LINC00659-exo group or sh-NC-exo group. sh-NC-exo was co-incubated with CRC and then transfected with miR-342-3p inhibitor or sh-ANXA2 plasmid were named as sh-NC-exo + in-miR-342-3p group or sh-NC-exo + sh-ANXA2 group. sh-LINC00659-exo was co-incubated with CRC and then transfected with miR-342-3p inhibitor or pcDNA3.1-ANXA2 plasmid were named as sh-LINC00659-exo + in-miR-342-3p group or sh-LINC00659-exo + pc-ANXA2 group.