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Antibodies to α sma and α tubulin

Manufactured by Merck Group

Antibodies to α-SMA and α-Tubulin are laboratory reagents used for the detection and quantification of specific target proteins in biological samples. The α-SMA antibody targets the alpha smooth muscle actin protein, while the α-Tubulin antibody targets the alpha-tubulin protein. These antibodies are commonly used in various research and diagnostic applications, such as Western blotting, immunohistochemistry, and flow cytometry.

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3 protocols using antibodies to α sma and α tubulin

1

Molecular Profiling of Signaling Pathways

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Antibodies to p-STAT3, STAT3, p-Smad3, Smad3, p-Src, Src, p-EGFR (Tyr-1068), p-EGFR (Tyr-845), p-AKT, AKT, Cyclin D1, Cyclin E, were purchased from Cell Signaling Technology (Danvers, MA). Antibodies to active caspase-3, PARP, fibronectin, Collagen 1(A2), PCNA, p21 and p27 were purchased from Santa Cruz, CA. The Tunel assay kit for apoptosis was purchased from Roche Life Science (Indianapolis, IN). Antibodies to α-SMA and α-Tubulin, and all other chemicals were obtained from Sigma (St. Louis, MO). Small interfering RNA (siRNA) specific for Src was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).
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2

Signaling Pathway Protein Analysis

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Antibodies to p-STAT3, STAT3, p-Smad3, Smad3, Smad7, p-PDGFR-β, PDGFR-β, p-EGFR (Tyr-845), p-NF-κB, NF-κB, p-53, fibronectin, were purchased from Cell Signaling Technology (Danvers, MA). Antibodies to Brd4, CD68, collagen 1(A2), c-Myc,MCP-1,GAPDH and the immunohistochemistry kit were purchased from Santa Cruz Biotechnology, CA. Small interfering RNA (siRNA) specific for Brd4 and Lipofectamine 2000 were purchased from Invitrogen (Carlsbad, CA). Antibodies to α-SMA and α-tubulin, and all other chemicals were obtained from Sigma (St. Louis, MO).
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3

Molecular Profiling of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to p-STAT3, STAT3, p-Smad3, Smad3, p-Src, Src, p-EGFR (Tyr-1068), p-EGFR (Tyr-845), p-AKT, AKT, Cyclin D1, Cyclin E, were purchased from Cell Signaling Technology (Danvers, MA). Antibodies to active caspase-3, PARP, fibronectin, Collagen 1(A2), PCNA, p21 and p27 were purchased from Santa Cruz, CA. The Tunel assay kit for apoptosis was purchased from Roche Life Science (Indianapolis, IN). Antibodies to α-SMA and α-Tubulin, and all other chemicals were obtained from Sigma (St. Louis, MO). Small interfering RNA (siRNA) specific for Src was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).
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