Rats were killed 7 days after ICH induction by intraperitoneal injection of chloral hydrate. Immunohistochemistry was performed as previously described (Lei et al., 2013 (link); Lapi et al., 2015 (link)). The primary antibody used was rabbit anti-rat CD31 (1:100, Abcam, USA). Total vessel densities were computed by counting 4 areas in 3 sections through the stroke region for each animal. Sections were stained with antibody against new vessel marker CD31; positive staining appeared brown. Standard quantitation was done as percent CD31-positive in the region bordering the hematoma.
Rabbit anti rat cd31
Manufactured by Abcam
Sourced in United States
Rabbit anti-rat CD31 is a primary antibody that recognizes the CD31 (PECAM-1) protein expressed on the surface of endothelial cells. CD31 is a cell adhesion molecule involved in the regulation of angiogenesis and vascular integrity. This antibody can be used in various immunoassays to detect and quantify CD31 in rat tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Rat anti mouse cd31, by Abcam (1 mentions)
Goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Ab185696, by Abcam (1 mentions)
Tritc conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Fitc conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Ab46154, by Abcam (1 mentions)
4 protocols using rabbit anti rat cd31
1
Immunohistochemical Analysis of Angiogenesis in ICH
2
Quantifying Capillary Density in Wound Healing
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Capillary densities in the healing wounds were quantitated by histological analysis. Wound samples were fixed with zinc chloride fixative (BD) for 24 h, then embedded in paraffin and sectioned at 4-µm intervals. Slides were de-paraffinized and hydrated. The slides were then placed in tris-buffered saline (TBS) (pH 7.5) for 5 min for pH adjustment. Endogenous peroxidase was blocked by 3% hydrogen peroxide/methanol bath for 20 min and followed by a distilled H2O rinse. Slides were blocked with 10% goat serum for 30 min at 37°C. The slides were then incubated for 60 min at room temperature with primary antibodies against CD31 (1:500 rabbit anti-rat CD31) and vascular endothelial growth factor (VEGF) 165 (VEGF165) (1:250; rabbit anti-rat VEGF) (both from Abcam, Cambridge, MA, U.S.A.). After primary antibody incubation, the sections were reacted with a secondary antibody (1:1000; goat anti-rat; Abcam). All the sections were counterstained with Hematoxylin.
3
Immunohistochemical Analysis of eNOS, iNOS, and nNOS
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Rabbit anti‐rat eNOS, rabbit anti‐rat iNOS, rabbit anti‐rat nNOS, rabbit anti‐rat Sphk1, mouse anti‐rat CD31, rabbit anti‐rat CD31, rabbit anti‐rat ERG, human anti‐rat Sphk1, and human anti‐rat iNOS were purchased from Abcam. Fluorescently conjugated secondary anti‐rabbit and anti‐mouse IgG were from Invitrogen. Sigma was the source of S1P, while an NO assay kit was from Nanjing Jiancheng Bioengineering Institute.
4
Immunohistochemical analysis of heart tissue
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Heart tissues were immersion-fixed in 4% paraformaldehyde and embedded in paraffin. Serial transverse sections (5 mm) were cut across the entire long axis of the heart and mounted on slides. After dewaxing, hydration and heat-induced antigen retrieval, heart specimens were incubated in a blocking buffer (PBS containing 5% goat serum and 0.1%Triton X-100) at room temperature for 1 h. Incubations in antibodies (diluted 1:250 in blocking buffer) were carried out at 4°C overnight for primary antibodies, and room temperature for 2 h for secondary antibodies. The primary antibodies used were: mouse antirat ɑ-SMA (sc-130616; 1:100, Santa Cruz), rabbit anti-rat VAChT (No.139 103; 1:250; Synaptic Systems); rabbit anti-rat CD31 (1:250; Abcam), rabbit-anti-rat VEGF-B (ab185696; 1:200; Abcam) and rabbit-anti-rat VEGF-A (ab46154; 1:200; Abcam). The secondary antibodies were horseradish peroxidase (HRP)-labeled goat anti-mouse IgG, goat-anti-rabbit IgG, FITC-conjugated anti-rabbit IgG, or TRITC-conjugated anti-mouse IgG (Jackson ImmunoResearch), respectively [20, 26] .
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