Jetoptimus system

Manufactured by Polyplus Transfection

Sourced in France

The JetOPTIMUS system is a laboratory equipment designed for the transfection of cells. It facilitates the introduction of genetic materials, such as DNA or RNA, into cells for research and experimental purposes.

Automatically generated - may contain errors

Lab products found in correlation

Microplate reader, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by PAN Biotech (1 mentions) Luciferase assay, by Promega (1 mentions) Ovcar3, by American Type Culture Collection (1 mentions) Jetprime system, by Polyplus Transfection (1 mentions) Image software, by Agilent Technologies (1 mentions) Elisa max deluxe set human ifn γ, by BioLegend (1 mentions) Gen5 microplate reader, by Agilent Technologies (1 mentions)

2 protocols using jetoptimus system

Neonatal and Adult Cardiomyocyte Isolation and Analysis

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Neonatal ventricular myocytes were isolated from 1 to 3-day-old neonatal Sprague-Dawley pups. The pups were sacrificed using anesthesia with 5% isoflurane for 3–5 min. The myocytes were cultured in M199 medium (Hyclone, Cytiva, China) supplemented with 10% FBS (PAN-Biotech GmbH, Germany). After 4 days, iNOS plasmids were transfected using the jetOPTIMUS system (Polyplus-Transfection SA, France).
Adult myocytes were isolated from the hearts of the mice using enzymatic digestion. The myocytes were allowed to adhere to culture dishes coated with laminin for 1 h in an incubator, then incubated with 5 μM 2-NBDG for 30 min. Fluorescence intensity was then detected using a microplate reader (Thermo Fisher Scientific, Inc., USA) at 485 nm.

Guo Y., Wen J., He A., Qu C., Peng Y., Luo S, & Wang X. (2022). iNOS contributes to heart failure with preserved ejection fraction through mitochondrial dysfunction and Akt S-nitrosylation. Journal of Advanced Research, 43, 175-186.

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Cytotoxicity and IFNγ Assay for CAR T Cells

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We plated 10⁵ target cells per well in a flat bottom 96 well plate in 100 μL culture media. The following day, we transfected the target cells with a luciferase expressing vector (Promega pGL4.51[luc2/CMV/Neo]) (RRID:Addgene_132962) using the JetPrime system (Polyplus Cat# 101000015) for tumor cells and the JetOptimus system (Polyplus Cat# 101000051) for primary healthy cells. The media was removed and replaced with fresh culture media 6-8 hours after transfection. The appropriate number of either OR2H1 CAR or mock transduced T cells were added the following day and co-cultured for 8 hours in the case of tumor cells and 24 hours for primary cultures (adipocytes, hepatocytes, and neurons). Cytotoxicity was measured via Luciferase Assay (Promega Cat# E1501). Cytotoxicity was calculated as (maximum viability control - individual well)/ (maximum viability control - maximum death control) *100 as a percentage.
In parallel, either H2009 (ATCC Cat# CRL-5911, RRID:CVCL_1514) or OVCAR3 (NCI-DTP Cat# OVCAR-3, RRID:CVCL_0465) cells were co-cultured with either OR2H1 CAR or mock transduced T cells at a ratio of 1:5. After co-culture of 8 hours, the supernatant was harvested and used for IFNγ detection using ELISA MAX™ Deluxe Set Human IFNγ (Biolegend 430101) and absorbance was measured at 450nm with Gen5 Microplate reader and Image Software (Biotek).

Martin A.L., Anadon C.M., Biswas S., Mine J.A., Handley K.F., Payne K.K., Mandal G., Chaurio R.A., Powers J.J., Sprenger K.B., Rigolizzo K.E., Innamarato P., Harro C., Mehta S., Perez B.A., Wenham R.M, & Conejo-Garcia J.R. (2022). Olfactory Receptor OR2H1 is an effective target for CAR T cells in human epithelial tumors. Molecular cancer therapeutics, 21(7), 1184-1194.

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