Lt α1β2

Manufactured by R&D Systems

Sourced in United Kingdom

LT-α1β2 is a recombinant protein that consists of the heterodimeric lymphotoxin-alpha (LT-α) and lymphotoxin-beta (LT-β) subunits. It is a member of the tumor necrosis factor (TNF) superfamily and plays a role in the development and organization of lymphoid tissues.

Automatically generated - may contain errors

Lab products found in correlation

B27 supplement, by Thermo Fisher Scientific (2 mentions) Recombinant mouse il 22, by Thermo Fisher Scientific (1 mentions) Advanced dmem f12, by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Noggin, by Thermo Fisher Scientific (1 mentions) N2 supplement, by Thermo Fisher Scientific (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Matrigel, by Corning (1 mentions) Tnf α, by R&D Systems (1 mentions)

3 protocols using lt α1β2

Modeling M-Cell Differentiation in Intestinal Organoids

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proximal small intestine was incubated with 2 mM EDTA/PBS for 30 min on ice. Then, intestinal crypts were isolated by vigorous pipetting and embedded in Matrigel (Corning). Crypts were cultured in Advanced DMEM/F12 (Thermo Fisher Scientific) containing 10 ng/ml EGF (PeproTech), R-spondin1 conditioning medium (provided by C.J. Kuo; Ootani et al., 2009 (link)), 100 ng/ml Noggin (PeproTech), B27 supplement (Thermo Fisher Scientific), N2 supplement (Thermo Fisher Scientific), and 1 mM N-acetylcysteine (Sigma-Aldrich). Recombinant glutathione S-transferase–mouse RANKL fusion protein (GST-RANKL; 500 ng/ml; Knoop et al., 2009 (link)) and LT α₁β₂ (1 µg/ml; R&D Systems) were used for the stimulation of organoids. Recombinant mouse IL-22 (100 ng/ml; PeproTech) was added into RANKL-stimulated organoids to evaluate the effect of IL-22 signaling on M cell differentiation.

Jinnohara T., Kanaya T., Hase K., Sakakibara S., Kato T., Tachibana N., Sasaki T., Hashimoto Y., Sato T., Watarai H., Kunisawa J., Shibata N., Williams I.R., Kiyono H, & Ohno H. (2017). IL-22BP dictates characteristics of Peyer’s patch follicle-associated epithelium for antigen uptake. The Journal of Experimental Medicine, 214(6), 1607-1618.

+ Open protocol

+ Expand

Isolation and Culture of Tonsil-Derived Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Stromal cells were obtained from human tonsils collected from children undergoing routine tonsillectomy, after informed consent as described previously (Ame-Thomas et al., 2007 (link)). Cells were cultured in αMEM media supplemented with 10% FBS at 37°C in a humid atmosphere with 5% CO² unless stated differently. For induction of FRC phenotype, cells were treated with TNF-α (10 ng/mL) and LT-α1β2 (100 ng/mL; R&D Systems, Abingdon, United Kingdom). On TopoChips, cells were seeded at a density of 10,000 cells/cm², using a seeding device (Unadkat et al., 2013 (link)) and cultured for 48 h (Supplementary Figure 2).

Vasilevich A.S., Mourcin F., Mentink A., Hulshof F., Beijer N., Zhao Y., Levers M., Papenburg B., Singh S., Carpenter A.E., Stamatialis D., van Blitterswijk C., Tarte K, & de Boer J. (2018). Designed Surface Topographies Control ICAM-1 Expression in Tonsil-Derived Human Stromal Cells. Frontiers in Bioengineering and Biotechnology, 6, 87.

+ Open protocol

+ Expand

Isolation and Differentiation of Tonsil Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

All tissues used for this study were obtained from subjects recruited under institutional review board approval and informed consent process according to the Declaration of Helsinki. Human stromal cells from SLO originated from pediatric patients undergoing routine tonsillectomy. Uncommitted tonsil stromal cells (TSCs) were obtained from Percoll-enriched cell fraction maintained in RPMI 1640-10% FCS, and were stimulated for 3 days by 20 ng/ml TNF-α and 100 ng/ml LT-α1β2 (RD Systems) to generate in vitro-differentiated FRC-like cells (FRCLs), as described previously (16 (link)). CXCR5^hiPD-1^hi CD4⁺CD3⁺CD25^- and CXCR5⁺PD-1^dimCD4⁺CD3⁺CD25^- were sorted (Supplemental Figure 1) and referred as GC-Tfh and R5-PD1^dim cells (4 (link)). It was assumed that few CD25^- Tfr cells were present in sorted samples. See Supplemental Materials and methods for details.

Misiak J., Jean R., Rodriguez S., Deleurme L., Lamy T., Tarte K, & Amé-Thomas P. (2020). Human Lymphoid Stromal Cells Contribute to Polarization of Follicular T Cells Into IL-4 Secreting Cells. Frontiers in Immunology, 11, 559866.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!