Sequencing grade modified trypsin

Manufactured by Worthington

Sourced in United States

Sequencing grade modified trypsin is a proteolytic enzyme used in protein sequencing and analysis. It cleaves peptide bonds on the C-terminal side of lysine and arginine residues, facilitating the breakdown of proteins into smaller fragments for further analysis.

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Trypsin (192 citations) Sequencing-grade trypsin (3 citations)

2 protocols using sequencing grade modified trypsin

Bovine Milk Protein Quantitation

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BSA was used
as the standard for protein quantitation and purchased from Sigma-Aldrich
(St. Louis, MO). Sodium bicarbonate (NaHCO₃), dithiotheritol
(DTT), iodoacetamide (IAA), ammonium hydroxide, sodium cyanoborohydride
(NaBH₃CN), formaldehyde (CH₂O), and formaldehyde-isotope
(¹³CD₂O) were from Sigma-Aldrich (St. Louis,
MO) as well. Sequencing grade modified trypsin was from Worthington
Biochemical Corporation (Freehold, NJ). Formic acid and acetonitrile
of HPLC grade were obtained from Merck (Darmstadt, Germany). Ultrapure
water was obtained by a Milli-Q Gradient water purification system
(Millipore, Bedford, MA). Raw bovine milk samples were provided by
Shiyun Lai in Beingmate Research Institute.

Chen Q., Jiang Y., Ren Y., Ying M, & Lu B. (2020). Peptide Selection for Accurate Targeted Protein Quantification via a Dimethylation High-Resolution Mass Spectrum Strategy with a Peptide Release Kinetic Model. ACS Omega, 5(8), 3809-3819.

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Quantitative Trastuzumab Analysis Protocol

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Trastuzumab standard (Herclon) with a concentration of 440 mg from Roche, India was used in this study. LC-MS grade acetonitrile, water, methanol from Fisher Scientific (Loughborough, UK); formic acid and ammonium bicarbonate from Fluka (St. Louis, MO, USA); Rapigest solution from Waters (Milford, MA, USA); dithiothreitol, and iodoacetamide from Sigma (St. Louis, MO, USA); sequencing grade modified trypsin from Worthington, Lakewood, NJ, USA were procured. Internal standard (ISTD) of trastuzumab signature peptides with a heavy labeled (¹³C¹⁵N-isotopically-KRFTISADTSK) was synthesized by Thermo (Waltham, MA, USA) and IYPTNGYTR was synthesized by commercial supplier, JPT peptide Inc. (Berlin, Germany).

Budhraja R.H., Shah M.A., Suthar M., Yadav A., Shah S.P., Kale P., Asvadi P., Valan Arasu M., Al-Dhabi N.A., Park C.G., Kim Y.O., Kim H.J., Agrawal Y.K, & Krovidi R.K. (2016). LC-MS/MS Validation Analysis of Trastuzumab Using dSIL Approach for Evaluating Pharmacokinetics. Molecules, 21(11), 1464.

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