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Mir 29a 3p mimic inhibitor nc

Manufactured by GenePharma
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Sourced in China

MiR-29a-3p mimic/inhibitor/nc is a laboratory product used for research purposes. It functions as a nucleic acid reagent that can modulate the expression of the miR-29a-3p microRNA. This product is intended for use in scientific experiments and investigations, without interpretation of its specific applications or intended uses.

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Lab products found in correlation

Pcdna 3.1 ltβr plasmids, by Genechem (3 mentions) Hoxa as3 speci c sirna sequences, by Genechem (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MiR-NC (532 citations) Mimic NC (350 citations) Mimics NC (68 citations) MiR-NC mimic (49 citations) MiR-NC inhibitor (33 citations) NC mimic/inhibitor (18 citations) MiR-29a mimic (11 citations) MiR-29a inhibitor (5 citations) MiR‐29a‐3p, (2 citations) MiR-29a (2 citations)

3 protocols using mir 29a 3p mimic inhibitor nc

1

Evaluating HOXA-AS3 Knockdown Efficiency

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Three different HOXA-AS3-specific siRNA sequences and a control sequence encoded in lentiviral vectors were purchased from GeneChem and were transduced into MKN-45 and SGC-7901 cells, after which qPCR was used to gauge relative knockdown efficiency. GAPDH was used for normalization purposes, with the 2−ΔΔCt method being used to assess levels of gene expression.
All miR-29a-3p mimic/inhibitor/nc were constructed from GenePharma (Suzhou, China), while pcDNA-3.1/LTβR plasmids and corresponding controls were obtained from GeneChem. Cells were transfected using Lipofectamine 2000 (ThermoFisher Scientific, MA) based on provided directions. At 48 h post-transfection, cells were utilized in downstream assays.
Qu F., Zhu B., Hu Y.L., Mao Q.S, & Feng Y. (2021). LncRNA HOXA-AS3 promotes gastric cancer progression by regulating miR-29a-3p/LTβR and activating NF-κB signaling. Cancer Cell International, 21, 118.
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2

Targeted Knockdown and Overexpression Assays

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Three different HOXA-AS3-speci c siRNA sequences and a control sequence encoded in lentiviral vectors were purchased from GeneChem and were transduced into MKN-45 and SGC-7901 cells, after which qPCR was used to gauge relative knockdown e ciency. GAPDH was used for normalization purposes, with the 2 -ΔΔCt method being used to assess levels of gene expression.
All miR-29a-3p mimic/inhibitor/nc were constructed from GenePharma (Suzhou, China), while pcDNA-3.1/LTβR plasmids and corresponding controls were obtained from GeneChem. Cells were transfected using Lipofectamine 2000 (ThermoFisher Scienti c, MA) based on provided directions. At 48 h posttransfection, cells were utilized in downstream assays.
Qu F., Zhu B., Hu Y., Mao Q., & Feng Y. (2021). LncRNA HOXA-AS3 promotes gastric cancer progression by regulating miR-29a-3p/LTβR and activating NF-κB signaling.
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3

Targeted Knockdown and Overexpression Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three different HOXA-AS3-speci c siRNA sequences and a control sequence encoded in lentiviral vectors were purchased from GeneChem and were transduced into MKN-45 and SGC-7901 cells, after which qPCR was used to gauge relative knockdown e ciency. GAPDH was used for normalization purposes, with the 2 -ΔΔCt method being used to assess levels of gene expression.
All miR-29a-3p mimic/inhibitor/nc were constructed from GenePharma (Suzhou, China), while pcDNA-3.1/LTβR plasmids and corresponding controls were obtained from GeneChem. Cells were transfected using Lipofectamine 2000 (ThermoFisher Scienti c, MA) based on provided directions. At 48 h posttransfection, cells were utilized in downstream assays.
Qu F., Zhu B., Hu Y., Mao Q., & Feng Y. (2020). LncRNA HOXA-AS3 Promotes Gastric Cancer Progression by Regulating miR-29a-3p/LTβR and Activating NF-κB Signaling.
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