Tropicamide ophthalmic solution 1

Mice were anesthetized (ketamine and xylazine) and pupils were dilated with one to two drops of Tropicamide Ophthalmic Solution 1% (Bausch & Lomb, Tampa, FL, USA). To create a consistent, optically transparent interface, an ocular demulcent solution containing hydroxypropyl methylcellulose ophthalmic (Gonak, Akorn, Lake Forest, IL, USA) was applied to the eye. Ultra-high resolution spectral domain optical coherence tomography (SD-OCT) imaging was performed on both eyes at the end of experiments (Bioptigen SD-OCT system, Research Triangle Park, Durham, NC, USA) as described [21 (link)]. In short, a series of 100 b-scans were collected, stacked, and aligned spatially to form a registered three-dimensional rendering of the retinal volume. By averaging 20 separate b-scans along the same horizontal axis and spatially aligning them, a high-resolution horizontal b-scan centered on the optic nerve head was obtained. The total retinal thickness, measured as the distance (in µm) between the retinal pigment epithelium and the inner limiting membrane, was calculated for each location. The Micron II retinal imaging microscope (Phoenix Research Laboratories, Inc., CA, USA) was used to capture fundus pictures.

Ketamine/xylazine was injected intraperitoneally to anesthetize mice. Pupils were dilated with one to two drops of Tropicamide Ophthalmic Solution 1% (Bausch & Lomb, Tampa, FL, USA). An ocular demulcent solution comprising hydroxypropyl methylcellulose ophthalmic (Gonak, Akorn, Lake Forest, IL, USA) was administered to the eye to create a consistent, optically transparent interface.
Ultra-high resolution spectral domain optical coherence tomography (SD-OCT) imaging was performed on both eyes at the end of experiments on day 7 (Bioptigen SD-OCT system, Research Triangle Park, Durham, NC, USA) as described [25 (link)]. The total retinal thickness—measured as a distance (in µm) between the inner limiting membrane and the retinal pigment epithelium—was determined for each location. The fundus images were acquired with a Micron II retinal imaging microscope (Phoenix Research Laboratories, Inc., Pleasanton, CA, USA).