To use optimized-releasing hydrogel, in vitro curcumin release studies were carried out using a dialysis method for six randomly selected hydrogels. In the next step, 1 ml of each gel was placed in a dialysis bag (D9527, Sigma), and then in 100 ml of a mixed methanol, double-distilled water solution (50:50 v/v).[18 ] The pH (7.4), temperature (37°C), and other conditions were kept steady over 21 days. Release test samples were taken and analyzed over 24, 48, and 72 h, and at 7, 14, and 21 days using a UV-vis Multi-Spec 1501 (Shimadzu, Japan) device that was calibrated using a mixture of methanol and water first. The curcumin detection limit wavelength was specified at 450-340 nm for a standard curcumin solution of 5% w/v.
D9527
Manufactured by Merck Group
D9527 is a laboratory equipment product. It is designed for scientific research and analysis purposes. The core function of D9527 is to facilitate the measurement and analysis of various samples and materials. Technical specifications and detailed information about the intended use or applications of this product are not available.
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Lab products found in correlation
3 protocols using d9527
1
Curcumin Release from Optimized Hydrogels
2
In Vitro Drug Release from Nanoparticles
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The
drug release studies were done in phosphate buffer of pH 7.4 in a
thermostatically shaking water bath at 37 ± 0.5 °C. To quantify
the amount of drug released, the nanoparticles of the respective composition
were taken in a dialysis membrane of 1200 molecular weight cutoff
(Sigma-Aldrich D-9527) and dialyzed against phosphate buffer of pH
7.4. The sample of nanoparticles without drug was used as a reference.
To monitor release of Td, the sample was taken at a regular time interval
and the same amount of buffer was replaced. The estimation of the
drug was done using the calibration curve of Td. The amount of Td
released at each interval was calculated from the OD recorded for
each solution at 271 nm using a UV–visible double beam spectrophotometer
(Shimadzu UV–1601 PC, Japan). The release studies were monitored
up to 48 h in duplicate where Mt is the amount of Td released at time t and M∞ is the total amount of
Td present in nanoparticles.
drug release studies were done in phosphate buffer of pH 7.4 in a
thermostatically shaking water bath at 37 ± 0.5 °C. To quantify
the amount of drug released, the nanoparticles of the respective composition
were taken in a dialysis membrane of 1200 molecular weight cutoff
(Sigma-Aldrich D-9527) and dialyzed against phosphate buffer of pH
7.4. The sample of nanoparticles without drug was used as a reference.
To monitor release of Td, the sample was taken at a regular time interval
and the same amount of buffer was replaced. The estimation of the
drug was done using the calibration curve of Td. The amount of Td
released at each interval was calculated from the OD recorded for
each solution at 271 nm using a UV–visible double beam spectrophotometer
(Shimadzu UV–1601 PC, Japan). The release studies were monitored
up to 48 h in duplicate where Mt is the amount of Td released at time t and M∞ is the total amount of
Td present in nanoparticles.
3
Determination of Encapsulated RSV Release
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Determination of free/unencapsulated RSV from these formulations was carried out by dialysis through semipermeable membrane (D9527, Sigma Aldrich). The release system was maintained under magnetic stirring (100 rpm) at room temperature and immersed in glass flasks containing 250 mL of water. An aliquot of 1 mL of RSV aqueous solution was monitored by UV-vis spectroscopy at different times (40, 60, 120, 180, 240 , 300, 360 min and 21 h). Results of encapsulation efficiency (E %) were calculated using the following equation (equation 1) and were expressed as the mean ± standard deviation (SD) of released RSV. It was calculated using the absorbance values of these solutions in the wavelength (λ) of 305 nm.
(
where CI is initial concentration and CF is final concentration determined after dialysis.
Experiments were performed at room temperature (27 °C) and at a pH of 6.5±0.1 [26] . Zupancic et al. [27] showed that RSV in aqueous solution was stable at room or body temperature at weakly acidic conditions of pH 6.8. Degradation of RSV is exponential above this pH value. The exposure to light and oxygen was avoided in order to maintain the stability of the trans isomer. The percentage of cumulative drug release (%CDR) profile of each delivery system was calculated. The graph is in the Supplementary Information (Figure S3).
(
where CI is initial concentration and CF is final concentration determined after dialysis.
Experiments were performed at room temperature (27 °C) and at a pH of 6.5±0.1 [26] . Zupancic et al. [27] showed that RSV in aqueous solution was stable at room or body temperature at weakly acidic conditions of pH 6.8. Degradation of RSV is exponential above this pH value. The exposure to light and oxygen was avoided in order to maintain the stability of the trans isomer. The percentage of cumulative drug release (%CDR) profile of each delivery system was calculated. The graph is in the Supplementary Information (Figure S3).
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