Log In Sign up
The largest database of trusted experimental protocols

Anti camp

Manufactured by LifeSpan BioSciences
Visit Supplier

Anti-CAMP is a laboratory reagent used to detect and quantify the presence of cyclic adenosine monophosphate (cAMP) in biological samples. cAMP is an important signaling molecule involved in various cellular processes. This product provides a reliable and sensitive method for researchers to measure cAMP levels in their experiments.

Automatically generated - may contain errors

Lab products found in correlation

Bis tris gel, by Thermo Fisher Scientific (2 mentions) Enhanced chemiluminescence, by Thermo Fisher Scientific (2 mentions) Anti human β actin, by Merck Group (1 mentions) Anti human β actin, by Abcam (1 mentions) Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions) Fluorescence microscope, by Zeiss (1 mentions) 4 6 diaminido 2 phenylindole dapi, by Vector Laboratories (1 mentions)

3 protocols using anti camp

1

Western Blot Analysis of CAMP

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western immunoblot analysis was performed, as described previously [7 (link)]. Briefly, cell lysates (25 μg), prepared in RIPA buffer, were resolved by electrophoresis on 4-12% Bis-Tris Gel (Invitrogen, Carlsbad, CA). Resultant bands blotted onto nitrocellulose membranes were probed with anti-CAMP (LifeSpan BioSciences, Seattle, WA), anti-human β-actin (Sigma-Aldrich, St. Louis, MO), and detected using enhanced chemiluminescence (Thermo Scientific, Waltham, MA).
Jeong S.K., Kim Y.I., Shin K.O., Kim B.W., Lee S.H., Jeon J.E., Kim H.J., Lee Y.M., Mauro T.M., Elias P.M., Uchida Y, & Park K. (2015). Sphingosine kinase 1 activation enhances epidermal innate immunity through sphingosine-1-phosphate stimulation of cathelicidin production. Journal of dermatological science, 79(3), 229-234.
+ Open protocol
+ Expand
2

Western Blot Analysis of CAMP Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western immunoblot analysis was performed as described previously [4 (link)]. Cell lysates (25 μg protein per lane), prepared in RIPA buffer, were resolved by electrophoresis on 4-12% Bis-Tris Gel (Invitrogen, Carlsbad, CA). Resultant bands blotted onto nitrocellulose membranes, were probed with anti-CAMP (LifeSpan BioSciences, Seattle, WA), anti-human β-actin (Abcam, Cambridge, MA), and detected using enhanced chemiluminescence (Thermo Scientific, Waltham, MA).
Park K., Kim Y.I., Shin K.O., Seo H.S., Kim J.Y., Mann T., Oda Y., Lee Y.M., Holleran W.M., Elias P.M, & Uchida Y. (2014). The dietary ingredient, genistein, stimulates cathelicidin antimicrobial peptide expression through a novel S1P-dependent mechanism. The Journal of nutritional biochemistry, 25(7), 734-740.
+ Open protocol
+ Expand
3

Genistein Modulates CAMP Localization in Keratinocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed, as described previously [21 (link)]. KC were treated with genistein or vehicle for 24 h. CAMP distribution was assessed using anti-CAMP (LifeSpan BioSciences) and anti-rabbit IgG conjugated with fluorescein isothiocyanate (Invitrogen). Cells were counterstained with the nuclear marker 4′,6-diaminido-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA) and images were viewed under a fluorescence microscope (Carl Zeiss, Thornwood, NY).
Park K., Kim Y.I., Shin K.O., Seo H.S., Kim J.Y., Mann T., Oda Y., Lee Y.M., Holleran W.M., Elias P.M, & Uchida Y. (2014). The dietary ingredient, genistein, stimulates cathelicidin antimicrobial peptide expression through a novel S1P-dependent mechanism. The Journal of nutritional biochemistry, 25(7), 734-740.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!