Heregulin

Manufactured by Merck Group

Sourced in United States

Heregulin is a lab equipment product manufactured by Merck Group. It functions as a recombinant human protein that can be used for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Forskolin, by Merck Group (7 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Cytosine arabinoside, by Merck Group (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) B27 supplement, by Thermo Fisher Scientific (2 mentions) Heregulin, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Collagenase, by Merck Group (1 mentions) Cytosine arabinoside arac, by Merck Group (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Anti thy1.1 antibody, by Merck Group (1 mentions) Cck 8 kit, by RiboBio (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Wst 1 kit, by Roche (1 mentions) Ag1478, by Bio-Techne (1 mentions) Cell culture media, by Thermo Fisher Scientific (1 mentions) 12 o tetradecanoylphorbol 13 acetate, by Merck Group (1 mentions) Protran nitrocellulose transfer membrane, by Cytiva (1 mentions) Cp 724714, by Selleck Chemicals (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Heregulin β1 (6 citations) Recombinant human heregulin-β1 (2 citations) Human heregulin-β1 (2 citations) Heregulin β1 (HRG; (2 citations) β-heregulin (2 citations) Heregulin beta-1 (2 citations)

11 protocols using heregulin

Heregulin Expression Evaluation in Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were cultured in 60-mm plates (Sumitomo Bakelite, Tokyo, Japan) at a density of 1.5×10⁶ cells per plate for 48 hours in RPMI medium supplemented with 2% FBS to assess heregulin expression in the cell lines. Then, the cell lysates were prepared for immunoblot assays [28 (link)] using antibodies against phosphorylated Akt, heregulin (both from Cell Signaling Technology, MA, USA) and β-actin (Sigma-Aldrich, MO, USA).
For confirmation of heregulin in cell culture supernatants, cells were seeded in 12-well plates at a density of 0.5×10⁶ cells per well in RPMI medium supplemented with 10% FBS. After incubation overnight, the medium was replaced with 1 ml of RPMI medium supplemented with 0.1% FBS. The cells were incubated for an additional 48 hours, and the culture supernatants were collected. The concentration of heregulin in cell culture supernatants was measured using the Human NRG1 beta 1 ELISA Kit (Abcam, Cambridge, UK) according to the manufacturer's instructions.

Nonagase Y., Yonesaka K., Kawakami H., Watanabe S., Haratani K., Takahama T., Takegawa N., Ueda H., Tanizaki J., Hayashi H., Yoshida T., Takeda M., Chiba Y., Tamura T., Nakagawa K, & Tsurutani J. (2016). Heregulin-expressing HER2-positive breast and gastric cancer exhibited heterogeneous susceptibility to the anti-HER2 agents lapatinib, trastuzumab and T-DM1. Oncotarget, 7(51), 84860-84871.

+ Open protocol

+ Expand

Isolation and Purification of Rat Schwann Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Schwann cells were isolated from the sciatic nerve of Sprague-Dawley rats aged 3 days [Animal Center of Chinese PLA 307 Hospital, Beijing, China; license No. SCXK-(Army)-2012-0004], Sciatic nerves were cut into small fragments and enzymatically dissociated in 1% collagenase (Sigma-Aldrich, St. Louis, MI, USA) for 30 minutes. Dulbecco's modified Eagle's medium/nutrient Mixture F-12 (DMEM/F12; Gibco-Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco-Invitrogen) was prepared as culture medium. The cell suspension was stirred, centrifuged and incubated in culture medium. Twenty-four hours later, the culture medium was replaced with culture media supplemented with 50 ng/mL cytosine arabinoside (Ara-C) (Sigma-Aldrich) to remove fibroblasts (Gu et al., 2012). After another 24 hours, the cells were incubated in culture medium containing 2 nM forskolin (Sigma-Aldrich) and 2 ng/mL heregulin (Sigma-Aldrich) to accelerate cell proliferation. Purity of cells was assessed by immunostaining with S100 (Kim et al., 2008). Cultures with purity above 95% were used for the following assays.

Zhou J.F., Wang Y.G., Cheng L., Wu Z., Sun X.D, & Peng J. (2016). Preparation of polypyrrole-embedded electrospun poly(lactic acid) nanofibrous scaffolds for nerve tissue engineering. Neural Regeneration Research, 11(10), 1644-1652.

+ Open protocol

+ Expand

Isolation and Purification of Schwann Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Schwann cells were harvested as previously described (Liu et al., 2019). Briefly, sciatic nerves were isolated from postnatal wild-type rats and green fluorescent protein-transgenic rats and digested with 3 mg/mL collagenase for 30 minutes and 0.125% trypsin for 10 minutes at 37°C. Isolated Schwann cells were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin and streptomycin for 24 hours. Cytosine arabinoside (10 µM; Sigma, St Louis, MO, USA) was added to the cell culture medium for 24 hours, and then 2 µM forskolin (Sigma) and 50 ng/mL heregulin (Sigma) were supplied to the culture media. Cells were then purified by the addition of anti-Thy1.1 antibody (1:1000; Sigma) and rabbit complement protein (1:3; Sigma) to remove contaminating fibroblasts.

Wang S., Gu M., Luan C.C., Wang Y., Gu X, & He J.H. (2021). Biocompatibility and biosafety of butterfly wings for the clinical use of tissue-engineered nerve grafts. Neural Regeneration Research, 16(8), 1606-1612.

+ Open protocol

+ Expand

Silk-Based Cell Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bombyx mori silk was purchased from Jiangsu and Zhejiang (China). Dulbecco’s modified Eagle’s medium and fetal bovine serum were obtained from Gibco (United States). Forskolin, heregulin, cytosine arabinoside, and 488-labeled goat anti-mouse IgG were obtained from Sigma-Aldrich (United States). Rabbit anti-S100 beta monoclonal antibody was obtained from Abcam. CCK-8 kit was purchased from Ribobio (Guangzhou, China).

Wang Y., Liang Y., Huang J., Gao Y., Xu Z., Ni X., Yang Y., Yang X, & Zhao Y. (2022). Proteomic Analysis of Silk Fibroin Reveals Diverse Biological Function of Different Degumming Processing From Different Origin. Frontiers in Bioengineering and Biotechnology, 9, 777320.

+ Open protocol

+ Expand

Signaling Pathway Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture media, phosphate buffered saline, and fetal bovine serum were obtained from Invitrogen (Gibco, Karlsruhe, Germany). Antibodies against Akt, phospho-Akt, p44/42 MAPK, phospho-p44/42 MAPK, actin, and anti-mouse IgG Alexa Fluor^® 647 (#4410S) were purchased from Cell Signaling (Danvers, MA, USA). Anti-HER3 purified antibody (clone 1B4C3) was purchased from BioLegend^®. Secondary antibodies were from Sigma–Aldrich (Steinheim, Germany). Protran Nitrocellulose Transfer membranes were purchased from Whatman (Dassel, Germany). The enhanced chemiluminescence systems (Super Signal West Femto Maximum Sensitivity Substrate and SuperSignal West Pico Chemiluminescent Substrate) were from Thermo-Scientific (Bonn, Germany). The WST-1 kit was from Roche Applied Science (Mannheim, Germany). The PCK inhibitor bisindolylmaleimide II (BIM II), the MET inhibitor PF04217903, and the HER1 inhibitor AG1478 were from Tocris (Wiesbaden, Germany). The HER2 inhibitor CP724714 was purchased from Selleckchem (Munich, Germany). Heregulin and 12-O-Tetradecanoylphorbol 13-acetate (PKC activator, PMA) were obtained from Sigma–Aldrich (Steinheim, Germany). The human phospho-MAPK Array Kit was from R&D (Minneapolis, MN, USA). All other chemicals used were purchased from Carl Roth (Karlsruhe, Germany) unless indicated otherwise.

Jenke R., Holzhäuser-Rein M., Mueller-Wilke S., Lordick F., Aigner A, & Büch T. (2020). SATB1-Mediated Upregulation of the Oncogenic Receptor Tyrosine Kinase HER3 Antagonizes MET Inhibition in Gastric Cancer Cells. International Journal of Molecular Sciences, 22(1), 82.

+ Open protocol

+ Expand

Generation of Human Astrocytes from iPSCs

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human astrocytes were generated from neuroepithelial-like stem (NES) cells, produced from human-induced pluripotent stem cells (iPSCs, Cntrl9 cell line) [25 (link), 26 (link)]. The NES cells were differentiated in Advanced DMEM/F12 (Thermo Fisher 12634-010) supplemented with 1% penicillin–streptomycin (Thermo Fisher 15140-122), 1% B27 supplement (Thermo Fisher, 11530536), 1% non-essential amino acids (Thermo Fisher, 11140050), and 1% l-glutamine (Thermo Fisher 25030-024). The following factors were added fresh to the medium just before use: basic fibroblast growth factor (bFGF) 10 ng/ml (Themo Fisher, 13256029), heregulin 10 ng/ml (Sigma Aldrich, SRP3055), activin A 10 ng/ml (Peprotech, 120-14E), and insulin-like growth factor 1 (IGF-1) 200 ng/ml (Sigma Aldrich, SRP3069). Additionally, 20 ng/ml ciliary neurotropic factor (CNTF; Thermo Fisher, PHC7015) was added to the medium the last 2 weeks of differentiation. Cells were seeded for experiment, at a concentration of 5000 cells/cm², directly after the differentiation protocol was completed.

Rostami J., Mothes T., Kolahdouzan M., Eriksson O., Moslem M., Bergström J., Ingelsson M., O’Callaghan P., Healy L.M., Falk A, & Erlandsson A. (2021). Crosstalk between astrocytes and microglia results in increased degradation of α-synuclein and amyloid-β aggregates. Journal of Neuroinflammation, 18, 124.

+ Open protocol

+ Expand

Neurotrophic Differentiation of Mesenchymal Progenitor Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

MPCs were seeded at 1 × 10³ cells/cm² on tissue culture plastic or NFs and differentiated neurotrophically using a modified 10-day protocol [27 (link), 29 (link), 42 (link)]. Twenty-four hours after seeding, MPCs were incubated with GM supplemented with 10 mM β-mercaptoethanol (Sigma) for 24 h, followed by 48 h in GM supplemented with 10 mM β-mercaptoethanol + 35 ng/ml retinoic acid (Sigma). For the 6 following days, MPCs were incubated with neurotrophic medium (DMEM/Ham’s F12 + PSF (Invitrogen) supplemented with 2% FBS, 2% B-27 (Invitrogen), 6 mg/ml retinoic acid, 1 ng/ml FGF-2 (Sigma), 10 ng/ml platelet-derived growth factor (PDGF; Sigma), 150 ng/ml heregulin (an isoform of neuregulin-1; Sigma), and 10 μM forskolin (Sigma)).
Following neurotrophic differentiation, MPCs (designated nMPCs) were washed with PBS and either lysed with TRiZol, or incubated with basal medium (see above) for 48 h to produce conditioned medium (nMPC-CM). For DRG coculture experiments, tissue culture plastic-cultured nMPCs were trypsinized and transferred to DRG-containing fibers at a concentration of 1 × 10³ cells/cm².

Zupanc H.R., Alexander P.G, & Tuan R.S. (2017). Neurotrophic support by traumatized muscle-derived multipotent progenitor cells: Role of endothelial cells and Vascular Endothelial Growth Factor-A. Stem Cell Research & Therapy, 8, 226.

+ Open protocol

+ Expand

Culturing Rat Schwann Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Schwann cells isolated from adult rat sciatic nerve (generous gift from Dr. Mary Bunge, University of Miami, Coral Gables, FL) were maintained in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 4mM L-glutamine, 100 μg/mL penicillin and 100 μg/mL streptomycin, supplemented with 2 μM forskolin (Sigma Aldrich), 10 μg/mL bovine pituitary extract (Sigma Aldrich), and 2 μM heregulin (Sigma Aldrich). Cells used were between passage 3 and 5. Dissociated Schwann cells were plated onto laminin-coated PAA substrates at a sparse density of 150 cells/cm² to minimize cell-cell interactions.

Evans E.B., Brady S.W., Tripathi A, & Hoffman-Kim D. (2018). Schwann cell durotaxis can be guided by physiologically relevant stiffness gradients. Biomaterials Research, 22, 14.

+ Open protocol

+ Expand

Differentiation of hAMSCs into SCLCs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

SCLCs were produced according to a previously published protocol with minor modifications [21 (link)]. Third-generation hAMSCs, at 80% confluence, were cultured for 24 h with DMEM low-sugar complete medium (11885084, Gibco, USA) containing 1 mM β-mercaptoethanol. The medium was then replaced with DMEM low-sugar complete medium containing 35 ng/ml all-trans retinoic acid (ATRA, R2625, Sigma, USA) and cultured for 72 h. The medium was then replaced with DMEM low-sugar complete medium containing 14 mmol/l forskolin (66575–29-9, Sigma, USA), l0 ng/ml basic fibroblast growth factor (AF-100-18B, PEPROTECH, USA), 5 ng/ml platelet-derived growth factor-aa (PDGF-AA, 07–1436, Sigma, USA) and 200 ng/ml heregulin (H7660, Sigma, USA) and cultured for 2 weeks. The medium was replaced every 3 days.

Hu T., Chang S., Qi F., Zhang Z., Chen J., Jiang L., Wang D., Deng C., Nie K., Xu G, & Wei Z. (2023). Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats. Burns & Trauma, 11, tkad013.

+ Open protocol

+ Expand

Schwann Cell Isolation and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sciatic nerve of suckling rats were cut into pieces and ground, hydrolyzed by 1% collagenase for 30 min, and then hydrolyzed in 0.25% trypsin (Roche, IN, USA) at 37 °C for 5–8 min. Digestion was terminated with high glucose DMEM containing 10% fetal bovine serum, followed by centrifugation at 1200 r/min for 5 min. Cells were cultured in DMEM containing 10% fetal bovine serum. After 16 h, the medium was added 10 mM cytosine arabinoside. Cells were incubated at 37 °C for 24 h to inhibit the proliferation of fibroblasts. Medium was changed to high glucose medium supplemented with 10% fetal bovine serum, 2 mM forskolin and 2 ng/ml heregulin (Sigma, MO, USA) to stimulate Schwann cell proliferation. The cell density occupied about 90% of the bottom area of the culture dish, and peeled with 0.25% trypsin. Schwann and Anti-thy 1.1 antibody (1:1000) were incubated at 4 °C for 2 h, and reacted with complement (Jackson, PA, USA) at 37 °C for 30 min to clear fibroblasts.

Yang P., Peng Y., Dai X., Jie J., Kong D., Gu X, & Yang Y. (2023). Bionic peptide scaffold in situ polarization and recruitment of M2 macrophages to promote peripheral nerve regeneration. Bioactive Materials, 30, 85-97.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!