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The AMTV 600 software is a comprehensive analytical tool designed for advanced microscopy techniques. It provides a range of advanced features and functionalities to facilitate the analysis and processing of microscopy data.

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2 protocols using amtv 600

1

Detailed Microscopic Analyses of Biological Samples

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Data are shown as means ± SEM. Analyses were performed with GraphPad Prism (v7.0, La Jolla, CA) using either Student’s t test (two-tailed with unequal variance) or Log-rank test for survival analysis. Images for transmission electron microscopy were obtained on a Philips EM300 electron microscope (FEI-Philips, Hillsboro, OR) with AMTV 600 software (Advanced Microscopy Techniques, Danvers, MA). Images for immunohistochemistical analysis were obtained with Zeiss Axiolmager Z2 and Axiocam 506 (San Diego, CA).
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2

Visualizing Phage Samples via Electron Microscopy

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PEG-precipitated phage samples as described above were visualized at The Rockefeller University Electron Microscopy Resource Center. Briefly, 10 µL of sample was spotted on glow discharged 200-mesh copper grids with carbon film (Electron Microscopy Sciences, Cat# CF200-Cu) and incubated for 2.5 min. Grids were then washed 2x in ddH2O, and stained with 2% 0.2 µm filtered uranyl acetate. Excess solution was wicked away and grids were allowed to dry at least 30 min before visualization. Grids were examined on the JEOL 100CX transmission electron microscope using AMT V600 software (Advanced Microscopy Techniques) and images captured at 33,000x and 50,000x magnification.
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