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Antibodies against occludin

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Antibodies against occludin are laboratory reagents used to detect and study the occludin protein in biological samples. Occludin is a tight junction protein that plays a role in the formation and regulation of cell-cell junctions. These antibodies can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to visualize and analyze the expression and localization of occludin in cells and tissues.

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2 protocols using antibodies against occludin

1

High-Fat Diet-Induced Metabolic Stress Study

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Japanese white rice was purchased from Matsushita rice-cleaning mill (Miyazaki, Japan). Yellow rice koji was purchased from Akita Konno Co., Ltd. (Akita, Japan). Lard and cellulose were purchased from Sigma-Aldrich Co., LLC. (Tokyo, Japan). Soybean oil, cholesterol, choline bitartrate, and methionine were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). AIN-93G mineral mix and AIN-93G vitamin mix were purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan). Corn starch was purchased from Sanwa Starch Co., Ltd. (Nara, Japan). Edible acid casein 30–60 Mesh was purchased from Meggle (Wasserburg am Inn., Germany). Sucrose was purchased from Hayashi Pure Chemical Ind., Ltd. (Osaka, Japan). Antibodies against occludin, NF-E2-related factor 2 (Nrf2), heme oxygenase 1 (HO1), NAD(P)H quinone dehydrogenase 1 (NQO1), and superoxide dismutase 2 (SOD2) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and β-actin was purchased from Cell Signaling Technology (Beverly, MA, USA).
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2

Endothelial Cell Culture and Analysis

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DMSO (Sigma-Aldrich) was used to dissolve non-water soluble reagents and as a vehicle control. Endothelial Cell Medium (ECM) which contains 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503) were purchased from ScienCell Research Laboratories. Antibodies against Occludin, Claudin5, CHOP, GRP78, and GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-p62 and microtubule-associated protein-1 light-chain 3 (LC3) were purchased from Cell Signaling Technologies (Danvers, MA, USA). Anti-β-catenin, P120-catenin, cleaved caspase-12, and PDI were purchased from Abcam. All chemicals including RA were from Sigma Chemical Company.
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