Id32c strips

In order to differentiate the bacterial species contaminating both the raw and diluted semen, each sample was plated onto Columbia agar with 5% (v/v) sheep blood and Mac-Conkey agar, respectively. The plates were incubated in aerobic conditions at 37 °C for 24 h. After this period, one colony of each morphotype was transferred onto tryptone soy agar and re-incubated for 24 h at 37 °C in order to obtain a fresh culture ready for identification. The identification of bacteria isolates was performed using Gram stain and ID32E, ID32GN, ID32STAPH, and ID32STREP (bioMérieux, Craponne, France). Filamentous fungi also called molds were identified on the basis of macroscopic and microscopic features using the primary cultures onto Sabouraud Chloramphenicol Agar. The yeasts were identified by biochemical tests using ID32C strips (bioMérieux, France) [41 ,42 ].

The determinations were performed on semen samples collected at T0 and T2. To identify bacterial contamination, each sample was placed on the plate onto the Columbia agar with 5% (v/v) sheep blood and Mac-Conkey agar, respectively. The plates were incubated in aerobic conditions at 37 °C for 24 h. After this period, one colony of each morphotype was transferred onto the tryptone soy agar and re-incubated for 24 h at 37 °C in order to obtain a fresh culture ready for identification. The identification of the bacteria isolates was performed using Gram stain and ID32E, ID32GN, ID32STAPH, and ID32STREP (bioMérieux, Craponne, France). Filamentous levura (fungi/yeasts) were identified on the basis of macroscopic and microscopic features using the primary cultures onto a Sabouraud Chloramphenicol Agar. The yeasts were identified by biochemical tests using ID32C strips (bioMérieux, France) [22 ,54 ].