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Anti fip200

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Anti-FIP200 is a primary antibody that recognizes the FIP200 (Focal Adhesion Kinase Family Interacting Protein of 200 kDa) protein. FIP200 is a key component of the ULK1 complex, which is involved in the initiation of autophagy. The Anti-FIP200 antibody can be used to detect and study the expression and localization of FIP200 in various biological samples.

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Lab products found in correlation

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Close spelling variants of this product

Anti-FIP200 (17250-1-AP; (4 citations) FIP200 (3 citations) Rabbit anti-FIP200 (3 citations) Anti-TM (2 citations)

3 protocols using anti fip200

1

Immunofluorescence Analysis of FIP200 in NPCs

Cited 1 time
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Immunofluorescence analysis was performed as previously described37 (link). First, 4% paraformaldehyde was used to fix NPCs, and then 0.5% Triton X-100 in PBS was used to permeabilize. The slides were washed in PBS and blocked with 2% bovine serum albumin (BSA) in PBS for 2 h at 37 °C, and then incubated with anti-FIP200 (1:50) (Proteintech) for 10 h. After washing twice, the slides were then incubated with goat anti-rabbit antibody (CST) at 37 °C for 1 h. Nuclei were then co-stained with 0.1 g/ml DAPI (Beyotime, Nantong, China), and images were captured under a microscope (Olympus, BX53; Melville, NY, USA).
Li G., Song Y., Liao Z., Wang K., Luo R., Lu S., Zhao K., Feng X., Liang H., Ma L., Wang B., Ke W., Yin H., Zhan S., Li S., Wu X., Zhang Y, & Yang C. (2020). Bone-derived mesenchymal stem cells alleviate compression-induced apoptosis of nucleus pulposus cells by N6 methyladenosine of autophagy. Cell Death & Disease, 11(2), 103.
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2

Comprehensive Antibody Collection for Cell Signaling

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Antibodies used in this study included anti-STING (13647; Cell Signaling Technology), anti-Flag M2 (F3165; Sigma-Aldrich), anti-HA (H3663; Sigma-Aldrich), anti-EGFP (GL-8; Clontech), anti-LC3 (7543; Sigma-Aldrich), anti-p62 (PM045; MBL), anti-STX17 (HPA001204; Sigma-Aldrich), anti-SNAP29 antibody (111303, SYSY, or sc-135564; Santa Cruz Biotechnology), anti-LAMP1(L1418; Sigma-Aldrich), anti-LAMP2 (sc-18822; Santa Cruz or L0668; Sigma-Aldrich), anti-Myc (9E10, DSHB), anti-WIPI2 (ab105459; Abcam), anti-FIP200 (17250; ProteinTech), anti-ATG16L (PM040; MBL), anti-α-Tubulin (E7; DSHB), anti-VAMP8 (ab76021; Abcam), anti-GABARAP (ab109364; Abcam), anti-AMPK (2532; Cell Signaling Technology), anti-p-AMPK T172 (2535; Cell Signaling Technology), anti-TBC1D1(66433; Cell Signaling Technology), anti-p-TBC1D1 Ser237(07-2268; Sigma-Aldrich), anti-Raptor (2280; Cell Signaling Technology), anti-p-Raptor Ser792 (2083; Cell Signaling Technology), anti-TSC2 (4308; Cell Signaling Technology), anti-p-TSC2 Ser1387 (5584; Cell Signaling Technology), anti-ACC (3662; Cell Signaling Technology), anti-p-ACC Ser79 (3661; Cell Signaling Technology), anti-Glut4 (GT-41-A; Alpha diagnostic international), anti-Laminin2 (L0663; Sigma-Aldrich), anti-CD36 (80080; Abcam), anti-Dystrophin (15277; Abcam), and LysoTracker (ENZ-51005; Enzo).
Rong Y., Zhang S., Nandi N., Wu Z., Li L., Liu Y., Wei Y., Zhao Y., Yuan W., Zhou C., Xiao G., Levine B., Yan N., Mou S., Deng L., Tang Z., Liu X., Kramer H, & Zhong Q. (2022). STING controls energy stress-induced autophagy and energy metabolism via STX17. The Journal of Cell Biology, 221(7), e202202060.
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3

Mitotic Checkpoint Protein Localization

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The antibodies used were anti-MAD1 (proteintech, #18322-1-AP; Santa Cruz, #sc-47746), anti-CENP-B (Santa Cruz, #sc-376283; #sc-22788), anti CDC20 (Santa Cruz, #sc-5296), anti-MAD2 (proteintech, #10337-1-AP; Covance, #PRB-452C-200), anti-BUBR1 (proteintech, #11504-2-AP), anti-cyclinB1 (abclonal, #A2056), anti-ZW10 (abcam, #ab21582), anti-Flag-tag (Sigma-Aldrich, #F1804), anti-ULK1 (Santa Cruz, #sc-33182; proteintech, #20986-1-AP), anti-Atg13 (CST, #13468S), anti-FIP200 (proteintech, #17250-1-AP), anti-Atg3 (MBL, #M133-3), anti-GFP-tag (Santa Cruz, #sc-9996), anti-His-tag (MBL, #D291-3), anti-BUB1 (proteintech, #13330-1-AP), anti-BUB3 (proteintech, #27073-1-AP), anti-H3S10 (abcam, #ab32017), anti-Knl1 (abclonal, A13108), anti-ROD (abclonal, A13064), anti-ZWILCH (proteintech, #14281-1-AP), anti-α-tubulin (CST, #3873T) and anti-actin (Santa Cruz, #sc-7210). A phospho (ph)-MAD1-S546 specific antibody was generated by Beijing Biodragon Immunotechnologies Co. Ltd.
Treatments included thymidine (Sigma, T9250), RO3306 (Selleck, S7747), Taxol (Santa Cruz, sc-201439), nocodazole (Selleck, S2775), vinblastine (Selleck, S4505).
Yuan F., Jin X., Li D., Song Y., Zhang N., Yang X., Wang L., Zhu W.G., Tian C, & Zhao Y. (2019). ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint. Nucleic Acids Research, 47(15), 8096-8110.
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