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Gsh assay kit

Manufactured by Enzo Life Sciences
Sourced in United States

The GSH Assay Kit is a laboratory equipment product designed for the quantitative determination of glutathione (GSH) levels in various biological samples. The kit utilizes an enzymatic recycling method to measure total GSH, providing a reliable and sensitive assay for research purposes.

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3 protocols using gsh assay kit

1

Glutathione Assay Protocol

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GSH assay kit was purchased from Enzo Life Sciences. The data were expressed as micromoles per milligram of protein.
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2

Renal Oxidative Stress Biomarkers

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Serum creatinine and BUN levels were analyzed using an automatic analyzer (Hitachi, Osaka, Japan). Renal MDA levels were measured using an MDA assay kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s protocol. Renal GSH and GSSG levels were assessed using a GSH assay kit (Enzo Life Sciences, Farmingdale, NY, USA) following the manufacturer’s instructions.
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3

Biochemical Markers in Disease

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Serum creatinine and blood urea nitrogen (BUN) levels were assessed using an automatic analyzer (Hitachi, Osaka, Japan). Serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were analyzed using ELISA kits (R&D Systems, Minneapolis, MN, USA). Malondialdehyde (MDA) levels were analyzed using a MDA assay kit (Sigma-Aldrich). Reduced glutathione (GSH) and oxidized glutathione (GSSG) levels were measured using a GSH assay kit (Enzo Life Sciences, Farmingdale, NY, USA). All analyses were conducted following the manufacturers' protocols.
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