Human gingival tissue samples were collected as surgical waste from clinics using NYU School of Medicine IRB approved protocol (Pushalkar et al., 2014 (link)). Samples were preserved in 10% neutral buffered formalin, followed by dehydration and then embedded in paraffin. The paraffin block was sectioned at 5 μm thickness. Sections were deparaffinized and stained on a Leica BondRX autostainer, according to the manufacturers’ instructions. In brief, sections underwent epitope retrieval for 20 min at 100°C with Leica Biosystems ER1 solution (pH 6.0, AR9961) followed by a 30 min incubation at 22°C with anti-GPR91 (Abcam, Cambridge, United Kingdom) diluted 1:200 in Leica diluent (Leica, Cat ARD1001EA) and subsequent detection using the BOND Polymer Refine Detection System (Leica, DS9800). Sections were counter-stained with hematoxylin scanned on a Hamamatsu Nanozoomer HT whole slide scanner and imported into the NYU Omero image database for viewing and annotation.
Anti gpr91
Manufactured by Abcam
Sourced in United Kingdom
Anti-GPR91 is a laboratory research tool used to detect and quantify the G-protein coupled receptor 91 (GPR91) in biological samples. GPR91 is a receptor involved in cellular signaling pathways. This product is intended for research use only and its specific applications should be evaluated by the user.
Automatically generated - may contain errors
2 protocols using anti gpr91
1
Immunohistochemical Analysis of GPR91 in Human Gingiva
2
Immunohistochemical Analysis of GPR91 in Human Gingiva
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Human gingival tissue samples were collected as surgical waste from clinics using NYU School of Medicine IRB approved protocol (Pushalkar et al., 2014 (link)). Samples were preserved in 10% neutral buffered formalin, followed by dehydration and then embedded in paraffin. The paraffin block was sectioned at 5 μm thickness. Sections were deparaffinized and stained on a Leica BondRX autostainer, according to the manufacturers’ instructions. In brief, sections underwent epitope retrieval for 20 min at 100°C with Leica Biosystems ER1 solution (pH 6.0, AR9961) followed by a 30 min incubation at 22°C with anti-GPR91 (Abcam, Cambridge, United Kingdom) diluted 1:200 in Leica diluent (Leica, Cat ARD1001EA) and subsequent detection using the BOND Polymer Refine Detection System (Leica, DS9800). Sections were counter-stained with hematoxylin scanned on a Hamamatsu Nanozoomer HT whole slide scanner and imported into the NYU Omero image database for viewing and annotation.
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