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Alexa fluor 555 donkey anti rabbit igg h l cross adsorbed

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Alexa Fluor 555 donkey anti-rabbit IgG (H+L) cross adsorbed is a secondary antibody conjugated with the Alexa Fluor 555 fluorescent dye. It is designed to detect and bind to rabbit primary antibodies, recognizing the heavy (H) and light (L) chains.

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Lab products found in correlation

Efluor 660 anti lyve1 monoclonal antibody, by Thermo Fisher Scientific (2 mentions) Chicken polyclonal anti gfp, by Aves Labs (1 mentions) Goat anti mouse vegfr3, by R&D Systems (1 mentions) Anti cd45 apc, by BioLegend (1 mentions) Rat monoclonal anti mouse cd31, by BD (1 mentions)

Close spelling variants of this product

Alexa Fluor 555 donkey anti-rabbit IgG (H+L) Anti-rabbit IgG H&L (Alexa Fluor® 555) Alexa Fluor 555 donkey anti-rabbit IgG Alexa Fluor 555 donkey anti-rabbit Alexa Fluor 555 anti-rabbit IgG Donkey anti-rabbit IgG (H + L) Alexa Fluor® donkey anti-rabbit IgG Alexa Fluor IgG H + L Alexa 555 donkey anti-rabbit Alexa Fluor 555 anti-rabbit

2 protocols using alexa fluor 555 donkey anti rabbit igg h l cross adsorbed

1

Immunofluorescence Staining of Mouse Tissues

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Primary antibodies included Living Colors Rabbit anti-dsRed Polyclonal Pan Antibody (1:200; Takara), Goat anti-Type 1 Collagen Polyclonal Antibody (1:200, Southern Biotech), Rat anti-CD326/EpCam Monoclonal Antibody (1:200, clone G8.8, BD Pharmingen), and Rabbit anti-DCAMKL1 Monoclonal Antibody (1:300, clone EPR6085, Abcam). The following species-specific secondary antibodies were used at 1:300 dilution: Alexa Fluor 488 donkey anti-goat IgG (H+L) cross-adsorbed (Thermo Fisher Scientific), Alexa Fluor 488 donkey anti-rat IgG (H+L) cross-adsorbed (Thermo Fisher Scientific), Alexa Fluor 555 donkey anti-rabbit IgG (H+L) cross adsorbed (Thermo Fisher Scientific), Alexa Fluor 647 donkey anti-goat IgG (H+L) cross adsorbed (Thermo Fisher Scientific), and Alexa Fluor 647 donkey anti-rabbit IgG (H+L) cross adsorbed (Thermo Fisher Scientific). The directly conjugated eFluor 660 anti-Lyve1 monoclonal antibody (clone ALY7, eBioscience) was used at 1:200 dilution. The directly conjugated AF488 anti-CD326/EpCam monoclonal antibody (clone G8.8, Biolegend) was used at 1:200 dilution.
O’Leary C.E., Sbierski-Kind J., Kotas M.E., Wagner J.C., Liang H.E., Schroeder A.W., de Tenorio J.C., von Moltke J., Ricardo-Gonzalez R.R., Eckalbar W.L., Molofsky A., Schneider C, & Locksley R.M. (2022). Bile acid-sensitive tuft cells regulate biliary neutrophil influx. Science immunology, 7(69), eabj1080.
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2

Multiparametric Immunofluorescence Imaging

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Primary antibodies used include Living Colors anti-DsRed Rabbit Polyclonal Pan Antibody (1:500; TaKaRa), Chicken Polyclonal anti-GFP (1:300, Aves labs), Rat Monoclonal anti-mouse CD31 (1:100, clone MEC 13.3, BD Pharmingen), Rat Monoclonal anti-Endomucine (1:200, clone V.7C7.1, Abcam), Rat Monoclonal anti mouse CD4 (1:100, clone RM4–5, Biolegend), Goat anti mouse VEGFR3 (1:300, clone Flt-4, R&D systems), Alexa Fluor 488 anti-aSMA monoclonal Antibody (1:200, clone 1A4, eBioscience), eFluor 660 anti-LYVE1 monoclonal Antibody (1:300, clone ALY7, eBioscience), Alexa Fluor 647 anti-CD4 (1:100, Clone RM4–5, Biolegend,), APC anti-CD45 (1:100, clone 30-F11, Biolegend). As necessary the following secondary antibodies were used at 1:400 dilution: Alexa Fluor 555 donkey anti-rabbit IgG (H+L) cross-adsorbed (ThermoFisher Scientific) and Alexa Fluor 647 donkey anti-rat IgG (H+L) cross-adsorbed (Abcam). CF™ 488A donkey anti-chicken IgY (H+L), cross-adsorbed (Sigma-Aldrich)
Cautivo K.M., Matatia P.R., Lizama C.O., Mroz N.M., Dahlgren M.W., Yu X., Sbierski-Kind J., Taruselli M.T., Brooks J.F., Wade-Vallance A., Caryotakis S.E., Chang A.A., Liang H.E., Zikherman J., Locksley R.M, & Molofsky A.B. (2022). Interferon gamma constrains type 2 lymphocyte niche boundaries during mixed inflammation. Immunity, 55(2), 254-271.e7.
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