Hmx hematology analyzer

Peripheral blood samples of the NHANES participants were analyzed at the Mobile Examination Centers (MEC) using a Beckman Coulter HMX Hematology Analyzer. Lymphocyte, neutrophil, monocyte, and platelet counts were measured via complete blood count, and were presented as ×10³ cells/μL. The SII and SIRI levels were calculated using the following formulas: platelet count × neutrophil count/lymphocyte count, monocyte count × neutrophil count/lymphocyte count, respectively. These values were expressed as ×10³ cells/μL based on previous studies (4 (link), 14 (link), 15 (link)). SII and SIRI were considered as exposure variables in this study.

Participants were asked to fast after 8:00 p.m., the evening before their clinic exam, and considered to be fasting after a minimum of a 10-h fast. Blood was drawn from participants in a supine position, using standard venipuncture technique, typically between 7:00 a.m. and 9:00 a.m. Hematology testing was performed using whole blood [Tyco Monoject, 15% ethylenediaminetetraacetic acid, EDTA, (K3)]. A CBC with differential was performed on EDTA whole blood using a Beckman Coulter HmX Hematology Analyzer as described previously (Sloan et al., 2015 (link)). Blood collection was performed following the exact same procedures in every subject. Hematology testing included estimation of intra-assay CV in ∼10% samples run in duplicate. The NLR was defined as the absolute neutrophil count by the absolute lymphocyte count from the CBC panel. Assessment of cognitive decline and dementia was performed by a panel blinded to the CBC/NLR results.

Complete blood count (CBC) was performed in routine practice at the College of Medicine and Public Health, Ubon Ratchathani University, by using the HmX Hematology Analyzer (Beckman Coulter, USA) within 24 h of blood collection. Hb analysis was done using capillary zone electrophoresis (Minicap: Sebia, Lisses, France). Serum ferritin (SF) was assayed using chemiluminescent immunoassay on the Syncron LXi^®725 Access^® Clinical system (Beckman Coulter, USA). Based the WHO criteria, Hb levels of less than 13.0 g/dL in male and 12.0 g/dL in female were classified as anemia and SF level lower than 15.0 µg/L was identified as ID¹⁵ . Measurement of CRP was performed by using CRP latex test kit (Plasmatec, UK). For globin gene genotyping, DNA was extracted from peripheral blood leukocytes using the GF1-blood DNA extraction kit (Vivantis Technologies Sdn Bhd, Selangor Darul Ehsan, Malaysia) and identifications of common α-thalassemia including α⁰-thalassemia (SEA & THAI deletions) and α⁺-thalassemia (− α^3.7 & − α^4.2) were routinely performed using the gap-PCR methods described elsewhere^{16 (link),17 (link)}. Hb Constant Spring and Hb Pakse′, the two α-hemoglobinopathies commonly found in the region were identified using a muliplex allele-specific PCR assay^{18 (link),19 (link)}. β-globin genotyping was performed using PCR based methods described elsewhere^{20 (link)}.