Commercially available analytical grade chemicals were used and not further purified. The chemical suppliers were: Sigma-Aldrich (Taufkirchen, Germany) and Merck (Darmstadt, Germany), unless otherwise indicated. Protected amino acids and resins were supplied from Novabiochem (Merck, Darmstadt, Germany) and IRIS Biotech (Marktredwitz, Germany).
The following RP-HPLC systems were used for analysis and purification: Agilent 1100 Series, equipped with a multi-wavelength-detector and Latek P402 (Latek Labortechnik-Geraete, Eppelheim, Germany) equipped with a HITACHI variable UV detector (absorbance measurement at 214 and 254 nm) and a gamma detector (Bioscan, Washington, USA). Both RP-HPLC systems were equipped with either an analytical Chromolith RP-18e (4.6 × 100 mm; Merck, Darmstadt, Germany) for analysis or a semi-preparative column Chromolith RP-18e (10 × 100 mm; Merck, Darmstadt, Germany) for purifications.
The MALDI-MS Daltonics Microflex (Bruker Daltonics, Bremen, Germany) system was used for mass spectrometry. Full-scan single mass spectra were obtained by scanning m/z = 400–4000.
Synthesis of PSMA PLT-TM is summarized inFigure 2 and described in detail in the following chapters:
The following RP-HPLC systems were used for analysis and purification: Agilent 1100 Series, equipped with a multi-wavelength-detector and Latek P402 (Latek Labortechnik-Geraete, Eppelheim, Germany) equipped with a HITACHI variable UV detector (absorbance measurement at 214 and 254 nm) and a gamma detector (Bioscan, Washington, USA). Both RP-HPLC systems were equipped with either an analytical Chromolith RP-18e (4.6 × 100 mm; Merck, Darmstadt, Germany) for analysis or a semi-preparative column Chromolith RP-18e (10 × 100 mm; Merck, Darmstadt, Germany) for purifications.
The MALDI-MS Daltonics Microflex (Bruker Daltonics, Bremen, Germany) system was used for mass spectrometry. Full-scan single mass spectra were obtained by scanning m/z = 400–4000.
Synthesis of PSMA PLT-TM is summarized in